Human IL-7

Human IL-7

IL-7 stands for interleukin 7. Human IL-7 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human IL-7 can be used for a variety of applications, including:
  • In vitro T cell expansion.
  • In vitro T cell priming.
  • In vitro differentiation of T cells and iNKT cells.
  • Investigation of IL-7 mediated signaling pathways.

Background information

IL-7 is a member of the type I cytokine family. The primary sources of IL-7 are non-hematopoietic stromal cells in bone marrow, thymus, and lymphoid organs and tissues. It is a pleiotropic cytokine with central roles in modulating T cell development and peripheral T cell homeostasis. IL-7 can act both as a T cell growth factor as well as a critical anti-apoptotic survival factor for naive and memory T cells. IL-7 is related to IL-2 and signals through a heterodimeric receptor composed of the common cytokine signaling γ-chain and IL-7 receptor α-chain.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of 2E8 cells (NIBSC 90/530)
  • premium grade: ≥ 5×
    10
    7
    U/mg
    (typical activity: ≥ 1.5×
    10
    8
    U/mg
    )
  • research grade: ≥ 2×
    10
    7
    U/mg
  • Selected references

    1. Engelmann S. et al. (2013) T cell-independent modulation of experimental autoimmune encephalomyelitis in ADAP-deficient mice. J. Immunol. 191(10): 4950-4959
    2. Laurenti, E. et al. (2015) CDK6 levels regulate quiescence exit in human hematopoietic stem cells. Cell Stem Cell 16(3): 302-313
    3. Velardi, E. et al. (2014) Sex steroid blockade enhances thymopoiesis by modulating Notch signaling. J. Exp. Med. 211(12): 2341-2349
    4. Dussiau C. et al. (2015) Targeting IRAK1 in T-cell acute lymphoblastic leukemia. Oncotarget 6: 18956-18965
    5. Ishihara, K. et al. (1991) Stromal-cell and cytokine-dependent lymphocyte clones which span the pre-B-to B-cell transition. Dev. Immunol. 1: 149-161
    6. Dietz, L. et al. (2010) Tracking human contact allergens: from mass spectrometric identification of peptide-bound reactive small chemicals to chemical-specific naive human T-cell priming. Toxicol. Sci. 117(2): 336-347
  • Certificates

    Please follow this
    link
    to search for Certificates of Analysis (CoA) by lot number.
Product options: 6
E. coli
10 µg
-
E. coli
25 µg
-
E. coli
10 µg
-
E. coli
25 µg
-
E. coli
100 µg
-
E. coli
1000 µg
-

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