Human IL-6

Human IL-6

IL-6 stands for interleukin 6. Human IL-6 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human IL-6 can be used for a variety of applications, including:
  • Induction of colony formation from hematopoietic progenitor cells in semi-solid medium.
  • Replacement of feeder cells in the preparation of murine and human hybridomas.
  • In vitro differentiation of TH 17 cells.

Background information

IL-6, originally identified as a B cell differentiation factor, is a multifunctional cytokine, which regulates immune responses, hematopoiesis, acute phase responses, and inflammatory reactions. It induces, for instance, the terminal maturation of activated B cells into antibody-secreting plasma cells and acts in synergy with IL-3 to support the proliferation of hematopoietic stem cells. IL-6 is produced by many cell types, such as monocytes, fibroblasts, endothelial cells, T cells, etc. Disturbed IL-6 production has been associated with pathological processes including inflammatory autoimmune diseases, such as rheumatoid arthritis and cancer.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of B9 hybridoma cells (NIBSC 89/548)
  • premium grade: ≥ 5×
    10
    7
    IU/mg
    (typical activity: ≥ 1.3×
    10
    8
    IU/mg
    )
  • research grade: ≥ 2×
    10
    7
    IU/mg
  • Selected References

    1. Kaebisch R. et al. (2014) Helicobacter pylori cytotoxin-associated gene A impairs human dendritic cell maturation and function through IL-10-mediated activation of STAT3. J. Immunol. 192: 316-323
    2. Laurenti, E. et al. (2015) CDK6 levels regulate quiescence exit in human hematopoietic stem cells. Cell Stem Cell 16(3): 302-313
    3. Ghirelli, C. et al. (2010) Systematic cytokine receptor profiling reveals GM-CSF as a novel TLR-independent activator of human plasmacytoid predendritic cells. Blood 115(24): 5037-5040
    4. Gaines-Das, R.E. and Poole, S. (1993) The international standard for interleukin-6. Evaluation in an international collaborative study. J. Immunol. Methods 160: 147-153
    5. Dighe N. et al. (2014) Long-term reproducible expression in human fetal liver hematopoietic stem cells with a UCOE-based lentiviral vector. PLoS One 9(8): e104805
    6. Wright, C. et al. (2012) Detection of multiple autoantibodies in patients with ankylosing spondylitis using nucleic acid programmable protein arrays. Mol. Cell. Proteomics 11(2): M9.00384
    7. Brault, J. et al. (2014)
      Optimized generation of functional neutrophils and macrophages from patient-specific induced pluripotent stem cells:
      ex vivo
      models of X
      0
      -linked, AR22
      0
      - and AR47
      0
      - chronic granulomatous diseases.
      Biores Open Access 3(6): 311-326
    8. Steinleitner, K. et al. (2012) EVI1 and MDS1/EVI1 expression during primary human hematopoietic progenitor cell differentiation into various myeloid lineages. Anticancer Res. 32(11): 4883-4889
Product options: 7

Product information

Size
Order no.
Price

Human IL-6, research grade

  • E. coli
  • For research use only
10 µg
130-095-365
EUR 135,00 

Human IL-6, research grade

  • E. coli
  • For research use only
25 µg
130-093-929
EUR 210,00 

Human IL-6, premium grade

  • E. coli
  • For research use only
10 µg
130-095-352
EUR 165,00 

Human IL-6, premium grade

  • E. coli
  • For research use only
25 µg
130-093-931
EUR 265,00 

Human IL-6, premium grade

  • E. coli
  • For research use only
100 µg
130-093-932
EUR 525,00 

Human IL-6, premium grade

  • E. coli
  • For research use only
500 µg
130-093-933
EUR 1.370,00 

Human IL-6, premium grade

  • E. coli
  • For research use only
1000 µg
130-093-934
EUR 2.220,00