Human IL-3

Human IL-3

IL-3 stands for interleukin 3. Human IL-3 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human IL-3 can be used for a variety of applications, including:
  • Induction of colony formation from hematopoietic progenitor cells in semi-solid medium in vitro , for example, CD34+ cells from umbilical cord blood2 .
  • In vitro differentiation studies, for example, of B lymphoid progenitors3 .
  • Cultivation of plasmacytoid dendritic cells4 .
  • In vitro expansion of hematopoietic stem cells.
  • Investigation of mast cell or basophil function, for example, basophil interaction with blood vessels5 .
  • Investigation of IL-3–mediated signaling pathways.

Background information

Interleukin 3 (IL-3) is a hematopoietic growth factor, which is produced mainly by activated T cells, but is also secreted by other cell types, including mast cells, eosinophils, and keratinocytes. The broad spectrum of biological activities of IL-3 includes the stimulation of the proliferation and differentiation of immature pluripotent hematopoietic stem cells and various lineage-committed progenitor cells, leading to the production of most of the major blood cell types. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils, and macrophages.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of TF-1 cells (NIBSC 91/510)
  • premium grade: ≥ 2×
    10
    6
    IU/mg
    (typical activity: ≥ 2.3×
    10
    6
    IU/mg
    )
  • research grade: ≥ 1×
    10
    6
    IU/mg
Product options: 7

Product information

Size
Order no.
Price

Human IL-3, research grade

  • E. coli
  • For research use only
10 µg
130-093-908
EUR 135,00 

Human IL-3, research grade

  • E. coli
  • For research use only
25 µg
130-093-909
EUR 210,00 

Human IL-3, research grade

  • E. coli
  • For research use only
4×25 µg
130-094-193
EUR 525,00 

Human IL-3, premium grade

  • E. coli
  • For research use only
10 µg
130-095-071
EUR 165,00 

Human IL-3, premium grade

  • E. coli
  • For research use only
25 µg
130-095-070
EUR 265,00 

Human IL-3, premium grade

  • E. coli
  • For research use only
100 µg
130-095-069
EUR 525,00 

Human IL-3, premium grade

  • E. coli
  • For research use only
1000 µg
130-095-068
EUR 2.220,00 

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