Human BMP-4

Human BMP-4

Recombinant human BMP-4 can be used for the differentiation of human embryonic and induced pluripotent stem cells, as in into cells of the endodermal and mesodermal lineage or into hematopoietic stem cells and progenitors. The bone morphogenetic protein (BMP)-4 belongs to the TGF-β superfamily and has diverse pivotal roles during bone and cartilage formation, tissue repair, or organ and neuronal development. BMP-4 recombinant protein can ideally be used for functional assays, cell culture, and differentiation studies.

Applications

Human BMP-4 can be used for a variety of applications, including:
  • Differentiation of human ES and iPS cells.
  • Maintenance of mouse ESCs under serum-free conditions.

Background information

Bone morphogenetic protein 4 (BMP-4) is a member of the bone morphogenetic protein family, which is part of the TGF-β superfamily. BMP-4 plays an important role in early differentiation of the embryo and in establishing of a dorsal-ventral axis. It is involved in bone and cartilage development and has been shown to play a role in the differentiation of sympathetic neurons. In synergy with Activin A it promotes the formation of definitive endoderm in human embryonic stem cells (ESCs). Furthermore, BMP-4 supports LIF (leukaemia inhibitory factor) as a positive factor for mouse ESC self-renewal and can replace the requirement of serum. The amino acid sequences of mature human BMP-4 shares 98% identity with mouse or rat BMP-4.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Biological activity

  • Induction of alkaline phosphatase assay using mouse C2C12 cells
  • premium grade: ≥ 8×
    10
    4
    U/mg
  • research grade: ≥ 4×
    10
    4
    U/mg
  • Selected references

    1. Cheng, H. et al. (2003) Osteogenic activity of the fourteen types of human bone morphogenetic proteins (BMPs). J Bone Joint Surg Am 85(A8): 1544-1552
  • Certificates

    Please follow this
    link
    to search for Certificates of Analysis (CoA) by lot number.
Product options: 6
Pichia pastoris
10 µg
EUR 155,00 
Pichia pastoris
25 µg
EUR 290,00 
Pichia pastoris
10 µg
EUR 190,00 
Pichia pastoris
25 µg
EUR 365,00 
Pichia pastoris
100 µg
EUR 840,00 
Pichia pastoris
1000 µg
EUR 3.280,00