Clone:
REA796
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, IF, IHC, MICS
Alternative names:
Ly-6C1, Ly-6C.2, Ly-6C2

Extended validation for Ly-6C Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA796
1G7.G10++
AL-21++
HK1.4+
RB6-8C5+
REA810+
Cells were incubated with an excess of purified unconjugated Ly-6C (REA796) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for Anti-Ly-6C. Splenocytes from C57BL/6 mice were stained with Anti-Ly-6C antibodies and with a suitable counterstaining. As a control, Anti-Ly-6C antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-Ly-6C. Splenocytes from C57BL/6 mice were stained with Anti-Ly-6C antibodies and with a suitable counterstaining. As a control, Anti-Ly-6C antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-Ly-6C. Splenocytes from C57BL/6 mice were stained with Anti-Ly-6C antibodies and with a suitable counterstaining. As a control, Anti-Ly-6C antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-Ly-6C. Splenocytes from C57BL/6 mice were stained with Anti-Ly-6C antibodies and with a suitable counterstaining. As a control, Anti-Ly-6C antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-Ly-6C. Splenocytes from C57BL/6 mice were stained with Anti-Ly-6C antibodies and with a suitable counterstaining. As a control, Anti-Ly-6C antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for Anti-Ly-6C. Splenocytes from C57BL/6 mice were stained with Anti-Ly-6C antibodies and with a suitable counterstaining. As a control, Anti-Ly-6C antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using Ly-6C (REA796). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using Ly-6C (REA796). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using Ly-6C (REA796). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for Ly-6C Antibody, anti-mouse, REAfinity™

Overview

Clone REA796 recognizes the mouse Ly-6C antigen, which is a 14–17 kDa GPI-linked cell surface antigen that belongs to the Ly-6 family of murine surface glycoproteins. It is expressed on monocytes in the bone marrow and shortly after their migration into the circulation. Moreover, Ly-6C is expressed on memory T cells and CD11
low
CD45R(B220)
+
mPDCA1
+
plasmacytoid dendritic cells (pDCs) in different organs.
Additional information: Clone REA796 displays negligible binding to Fc receptors.

Alternative names

Ly-6C1, Ly-6C.2, Ly-6C2

Detailed product information

Technical specifications

CloneREA796
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenLy-6C
Alternative names of antigenLy-6C1, Ly-6C.2, Ly-6C2
Molecular mass of antigen [kDa]9
Distribution of antigenmonocytes, bone marrow
Entrez Gene ID100041546
RRIDAB_2652801, AB_2652802, AB_2652803, AB_2652804, AB_2652805, AB_2652806, AB_2652808, AB_2652809, AB_2652810, AB_2652811, AB_2652812, AB_2652814, AB_2652815, AB_2652816, AB_2652817, AB_2652800

References for Ly-6C Antibody, anti-mouse, REAfinity™

Publications

  1. Shevach, E. M. and Korty, P. E. (1989) Ly-6: a multigene family in search of a function. Immunol. Today 10: 195-220
  2. Schlueter, A. J. et al. (1997) Distribution of Ly-6C on lymphocyte subsets: I. Influence of allotype on T lymphocyte expression. J. Immunol. 158: 4211-4222
  3. Hänninen, A. et al. (1997)
    Ly-6C regulates endothelial adhesion and homing of CD8
    +
    T cells by activating integrin-dependent adhesion pathways.
    Proc. Natl. Acad. Sci. U.S.A. 94: 6898-6903
  4. Curtsinger, J. M. et al. (1998)
    CD8
    +
    memory T cells (CD44
    high
    , Ly-6C
    +
    ) are more sensitive than naive cells to (CD44
    low
    , Ly-6C
    -
    ) to TCR/CD8 signaling in response to antigen.
    J. Immunol. 160: 3236-3243
  5. Sunderkötter, C. et al. (2004) Subpopulations of mouse blood monocytes differ in maturation stage and inflammatory response. J. Immunol. 172: 4410-4417

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