The Liver Perfusion Kit has been developed for the gentle, rapid, and efficient generation of single-cell suspensions from rodent liver based on
ex vivo
perfusion using gentleMACS Perfusion Technology. It is optimized to obtain a high yield of parenchymal rodent liver cells, i.e., hepatocytes, while preserving most cell surface epitopes. Intact viable hepatocytes can be subsequently cultured, and used for downstream analysis such as genetic and proteomic analysis or functional assays. Furthermore, non-parenchymal liver cells are also retrieved after perfusion and can be used for further downstream

Specifications for Liver Perfusion Kit, mouse and rat

Overview

The Liver Perfusion Kit has been developed for the gentle, rapid, and efficient generation of single-cell suspensions from rodent liver based on
ex vivo
perfusion using gentleMACS Perfusion Technology. It is optimized to obtain a high yield of parenchymal rodent liver cells, i.e., hepatocytes, while preserving most cell surface epitopes. Intact viable hepatocytes can be subsequently cultured, and used for downstream analysis such as genetic and proteomic analysis or functional assays. Furthermore, non-parenchymal liver cells are also retrieved after perfusion and can be used for further downstream applications such as cell separation, cell culture or cell analysis.

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