Clone:
REA762
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
Fex, GPR49, GPR67, GRP49, HG38

Specifications for LGR5 Antibody, anti-human, REAfinity™

Overview

Clone REA762 recognizes the human leucine-rich repeat-containing G-protein–coupled receptor 5 (LGR5) but not with its close homologues LGR4 or LGR6. LGR5 associates with Wnt-receptors and act as R-spondin receptor thereby playing a central role in the modulation of Wnt/β-catenin signaling in normal and neoplastic stem cells. Initially described as a highly specific marker for stem cells in the small intestine, colon, hair follicle, stomach, and during kidney development, LGR5 positive cells were also shown to be crucial during the development and progression of cancer. It was shown that LGR5 positive crypt stem cells are the cells-of-origin in intestinal cancer and that CSCs in human colorectal cancer can be identified and isolated based on LGR5 expression.
Additional information: Clone REA762 displays negligible binding to Fc receptors.

Alternative names

Fex, GPR49, GPR67, GRP49, HG38

Detailed product information

Technical specifications

CloneREA762
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenLGR5
Alternative names of antigenFex, GPR49, GPR67, GRP49, HG38
Molecular mass of antigen [kDa]98
Distribution of antigenstem cells, cancer stem cells
Entrez Gene ID8549
RRIDAB_2652609, AB_2652610, AB_2652611, AB_2652612, AB_2652613, AB_2652614, AB_2652615, AB_2652616, AB_2652617, AB_2652618, AB_2652619, AB_2652608

References for LGR5 Antibody, anti-human, REAfinity™

Publications

  1. Barker, N. et al. (2007) Identification of stem cells in small intestine and colon by marker gene Lgr5. Nature 449(7165): 1003-1007
  2. Barker, N. et al. (2012)
    LGR5
    +
    stem/progenitor cells contribute to nephron formation during kidney development.
    Cell Rep 2: 540-552
  3. Jaks, V. et al. (2008) Lgr5 marks cycling, yet long-lived, hair follicle stem cells. Nat. Genet. 40: 1291-1299
  4. Barker, N. et al. (2009) Crypt stem cells as the cells-of-origin of intestinal cancer. Nature 457: 608-611
  5. Barker, N. et al. (2010)
    Lgr5
    +
    stem cells drive self-renewal in the stomach and build long-lived gastric units
    in vitro
    .
    Cell Stem Cell 6(1): 25-36
  6. Carmon, K. S. et al. (2011) R-spondins function as ligands of the orphan receptors LGR4 and LGR5 to regulate Wnt/β-catenin signaling. Proc. Natl. Acad. Sci. U.S.A. 108: 11452-11457
  7. de Lau, W. et al. (2011) Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling. Nature 476(7360): 293-297
  8. Kemper, K. et al. (2012) Monoclonal antibodies against lgr5 identify human colorectal cancer stem cells. Stem Cells 30(11): 2378-2386
  9. Dame, M. K. et al. (2014) Human colonic crypts in culture: segregation of immunochemical markers in normal versus adenoma-derived. Lab. Invest. 94(2): 222-234

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