Clone:
DA03-22H2.8
Type of antibody:
Primary antibodies
Isotype:
rat IgG2b
Applications:
FC
Alternative names:
Fex, GPR49, GPR67, GRP49, HG38

Specifications for LGR5 Antibody, anti-human

Overview

Clone DA03-22H2.8 reacts with the human leucine-rich repeat-containing G-protein–coupled receptor 5 (LGR5) but not with its close homologues LGR4 or LGR6. LGR5 associates with Wnt-receptors and act as R-spondin receptor thereby playing a central role in the modulation of Wnt/β-catenin signaling in normal and neoplastic stem cells. Initially described as a highly specific marker for stem cells in the small intestine, colon, hair follicle, stomach, and during kidney development, LGR5 positive cells were also shown to be crucial during the development and progression of cancer. It was shown that LGR5 positive crypt stem cells are the cells-of-origin in intestinal cancer and that CSCs in human colorectal cancer can be identified and isolated based on LGR5 expression.

Alternative names

Fex, GPR49, GPR67, GRP49, HG38

Detailed product information

Technical specifications

CloneDA03-22H2.8
Clonalitymonoclonal
Isotyperat IgG2b
Isotype controlIsotype Control Antibody, rat IgG2b
Hostrat
Type of antibodyPrimary antibodies
Specieshuman
AntigenLGR5
Alternative names of antigenFex, GPR49, GPR67, GRP49, HG38
Distribution of antigenstem cells, cancer stem cells
RRIDAB_2727925, AB_2652630, AB_2652622, AB_2652623, AB_2652624, AB_2652625, AB_2652626, AB_2652627, AB_2652628, AB_2652629, AB_2727969

Resources for LGR5 Antibody, anti-human

References for LGR5 Antibody, anti-human

Publications

  1. Barker, N. et al. (2007) Identification of stem cells in small intestine and colon by marker gene Lgr5. Nature 449(7165): 1003-1007
  2. Barker, N. et al. (2009) Crypt stem cells as the cells-of-origin of intestinal cancer. Nature 457: 608-611
  3. de Lau, W. et al. (2011) Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling. Nature 476(7360): 293-297
  4. Barker, N. et al. (2010)
    Lgr5
    +
    stem cells drive self-renewal in the stomach and build long-lived gastric units
    in vitro
    .
    Cell Stem Cell 6(1): 25-36
  5. Carmon, K. S. et al. (2011) R-spondins function as ligands of the orphan receptors LGR4 and LGR5 to regulate Wnt/β-catenin signaling. Proc. Natl. Acad. Sci. U.S.A. 108: 11452-11457
  6. Dame, M. K. et al. (2014) Human colonic crypts in culture: segregation of immunochemical markers in normal versus adenoma-derived. Lab. Invest. 94(2): 222-234
  7. Jaks, V. et al. (2008) Lgr5 marks cycling, yet long-lived, hair follicle stem cells. Nat. Genet. 40: 1291-1299
  8. Kemper, K. et al. (2012) Monoclonal antibodies against lgr5 identify human colorectal cancer stem cells. Stem Cells 30(11): 2378-2386
  9. Barker, N. et al. (2012)
    LGR5
    +
    stem/progenitor cells contribute to nephron formation during kidney development.
    Cell Rep 2: 540-552

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