The Indirect CD34 MicroBead Kit was developed for the isolation of CD34
+
precursor cells, using a two-step procedure involving indirect magnetic labeling from
  • peripheral blood
  • bone marrow
  • leukapheresis product
  • cord blood
  • differentiated-ES and iPS cells
Even faster, one-step isolation of CD34
+
cells can be achieved with the CD34 MicroBead Kit.

Data and images for Indirect CD34 MicroBead Kit, human

Figures

Figure 1

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May-Grünwald-Giemsa–stained CD34
+
cells isolated from bone marrow aspirate using the Indirect CD34 MicroBead Kit, an MS Column and a MiniMACS™ Separator.

Figure 1

May-Grünwald-Giemsa–stained CD34
+
cells isolated from bone marrow aspirate using the Indirect CD34 MicroBead Kit, an MS Column and a MiniMACS™ Separator.

Specifications for Indirect CD34 MicroBead Kit, human

Overview

The Indirect CD34 MicroBead Kit was developed for the isolation of CD34
+
precursor cells, using a two-step procedure involving indirect magnetic labeling from
  • peripheral blood
  • bone marrow
  • leukapheresis product
  • cord blood
  • differentiated-ES and iPS cells
Even faster, one-step isolation of CD34
+
cells can be achieved with the CD34 MicroBead Kit.

Detailed product information

Background information

CD34 is a well-established marker of human hematopoietic stem and progenitor cells and additionally expressed on hemangioblasts
1,2
, endothelial progenitor cells
1,3-5
, and mature endothelial cells
4
.

Detailed separation procedure

First, cells are labeled with the hapten-modified CD34 primary antibody QBEND/10
6
. In a second step, antibody-labeled cells are magnetically labeled with Anti-Hapten MicroBeads.
Control staining of cells isolated with the Indirect CD34 MicroBead Kit requires a monoclonal CD34 antibody other than QBEND/10, e.g. clone AC136

Downstream applications

  • Transplantation of CD34+ cells into humanized mouse models
  • Expansion and differentiation in culture
  • Reprogramming of CD34+ HSCs into iPS cells
  • Differentiation of ES or iPS cell-derived CD34+ -derived precursors into endothelial or hematopoietic lineages
  • Evaluation of differentiation potential in CFU assays
  • Characterization of surface marker expression by flow cytometry.

Columns

MS, LS, XS, or autoMACS
®
Columns.

References for Indirect CD34 MicroBead Kit, human

Publications

  1. Wang, Z. Z. et al. (2007) Endothelial cells derived from human embryonic stem cells form durable blood vessels in vivo. Nat. Biotechnol. 25: 317-318
  2. Loges, S. et al. (2004) Identification of the adult human hemangioblast. Stem Cells Dev. 13: 229-242
  3. Peichev, M. et al. (2000)
    Expression of VEGFR-2 and AC133 by circulating human CD34
    +
    cells identifies a population of functional endothelial precursors.
    Blood 95: 952-958
  4. Rafii, S. and Lyden, D. (2003) Therapeutic stem and progenitor cell transplantation for organ vascularization and regeneration. Nat Med 9: 702-712
  5. Valgimigli, M. et al. (2004)
    CD34
    +
    and endothelial progenitor cells in patients with various degrees of congestive heart failure.
    Circulation 110: 1209-1212
  6. Lanza, F. et al. (2001) Structural and functional features of the CD34 antigen: an update. J. Biol. Regul. Homeost. Agents 15: 1-13

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