The IL-5 Secretion Assay was developed for the sensitive detection of human IL-5-secreting cells.

Data and images for IL-5 Secretion Assay – Detection Kit, human

Figures

Figure 1

Human PBMCs were stimulated with 1 µg/mL SEB for 16 hours. Coexpression of IL-5 and IFN-γ was analyzed by using a combination of the IL-5 Secretion Assay - Detection Kit (APC) and the IFN-γ Secretion Assay - Detection Kit (PE). The plots show IFN-γ and IL-5 secretion by viable CD4
+
T cells.
Stimulated sample
Unstimulated control
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Figure 1

Human PBMCs were stimulated with 1 µg/mL SEB for 16 hours. Coexpression of IL-5 and IFN-γ was analyzed by using a combination of the IL-5 Secretion Assay - Detection Kit (APC) and the IFN-γ Secretion Assay - Detection Kit (PE). The plots show IFN-γ and IL-5 secretion by viable CD4
+
T cells.
View details

Figure 1

Human PBMCs were stimulated with 1 µg/mL SEB for 16 hours. Coexpression of IL-5 and IFN-γ was analyzed by using a combination of the IL-5 Secretion Assay - Detection Kit (APC) and the IFN-γ Secretion Assay - Detection Kit (PE). The plots show IFN-γ and IL-5 secretion by viable CD4
+
T cells.

Specifications for IL-5 Secretion Assay – Detection Kit, human

Overview

The IL-5 Secretion Assay was developed for the sensitive detection of human IL-5-secreting cells.

Detailed product information

Background information

Interleukin-5 (IL-5) is predominantly produced by CD4
+
T cells. IL-5 secretion has also been reported for γ/δ T cells, mast cells, NK cells, NKT cells, and eosinophils.
The cytokine was originally identified by its activity as a B cell growth factor and IgA-enhancing factor. Furthermore, IL-5 regulates growth, differentiation, activation, and survival of eosinophils from bone marrow into peripheral circulation (e.g. into lung following allergen exposure). It is involved in a range of allergic reactions and mediates immune reactions against parasites.
They can also be used for two-color cytokine analysis in combination with the IFN-γ, IL-2, IL-4, or IL-10 Secretion Assay - Detection Kits. The IL-5 Secretion Assay - Detection Kit (APC) can also be used for staining of IL-5-secreting cells in combination with peptide-MHC tetramers conjugated to R-phycoerythrin (PE).

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