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Human PBMCs were stimulated for 1 hour with CytoStim or left untreated. The responding cells were stained and isolated according to secretion of IL-5 using the IL-5 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 266 IL-5-secreting CD4 + cells were enriched per 10 6 CD4 + T cells by using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, one IL-5-secreting CD4 + T cell was detected per 10 6 CD4 + T cells after enrichment. |
Stimulated sample | |
Before enrichment | After enrichment |
IL-5 Secretion Assay – Cell Enrichment and Detection Kit (PE), humanFigure 1Human PBMCs were stimulated for 1 hour with CytoStim or left untreated. The responding cells were stained and isolated according to secretion of IL-5 using the IL-5 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 266 IL-5-secreting CD4 + cells were enriched per 10 6 CD4 + T cells by using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, one IL-5-secreting CD4 + T cell was detected per 10 6 CD4 + T cells after enrichment. | IL-5 Secretion Assay – Cell Enrichment and Detection Kit (PE), humanFigure 1Human PBMCs were stimulated for 1 hour with CytoStim or left untreated. The responding cells were stained and isolated according to secretion of IL-5 using the IL-5 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 266 IL-5-secreting CD4 + cells were enriched per 10 6 CD4 + T cells by using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, one IL-5-secreting CD4 + T cell was detected per 10 6 CD4 + T cells after enrichment. |
Unstimulated control | |
Before enrichment | After enrichment |
IL-5 Secretion Assay – Cell Enrichment and Detection Kit (PE), humanFigure 1Human PBMCs were stimulated for 1 hour with CytoStim or left untreated. The responding cells were stained and isolated according to secretion of IL-5 using the IL-5 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 266 IL-5-secreting CD4 + cells were enriched per 10 6 CD4 + T cells by using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, one IL-5-secreting CD4 + T cell was detected per 10 6 CD4 + T cells after enrichment. | IL-5 Secretion Assay – Cell Enrichment and Detection Kit (PE), humanFigure 1Human PBMCs were stimulated for 1 hour with CytoStim or left untreated. The responding cells were stained and isolated according to secretion of IL-5 using the IL-5 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 266 IL-5-secreting CD4 + cells were enriched per 10 6 CD4 + T cells by using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, one IL-5-secreting CD4 + T cell was detected per 10 6 CD4 + T cells after enrichment. |
Human PBMCs were stimulated for 1 hour with CytoStim or left untreated. The responding cells were stained and isolated according to secretion of IL-5 using the IL-5 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 266 IL-5-secreting CD4 + cells were enriched per 10 6 CD4 + T cells by using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, one IL-5-secreting CD4 + T cell was detected per 10 6 CD4 + T cells after enrichment. |
Stimulated sample | |
Before enrichment | After enrichment |
IL-5 Secretion Assay – Cell Enrichment and Detection Kit (PE), humanFigure 1Human PBMCs were stimulated for 1 hour with CytoStim or left untreated. The responding cells were stained and isolated according to secretion of IL-5 using the IL-5 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 266 IL-5-secreting CD4 + cells were enriched per 10 6 CD4 + T cells by using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, one IL-5-secreting CD4 + T cell was detected per 10 6 CD4 + T cells after enrichment. | IL-5 Secretion Assay – Cell Enrichment and Detection Kit (PE), humanFigure 1Human PBMCs were stimulated for 1 hour with CytoStim or left untreated. The responding cells were stained and isolated according to secretion of IL-5 using the IL-5 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 266 IL-5-secreting CD4 + cells were enriched per 10 6 CD4 + T cells by using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, one IL-5-secreting CD4 + T cell was detected per 10 6 CD4 + T cells after enrichment. |
Unstimulated control | |
Before enrichment | After enrichment |
IL-5 Secretion Assay – Cell Enrichment and Detection Kit (PE), humanFigure 1Human PBMCs were stimulated for 1 hour with CytoStim or left untreated. The responding cells were stained and isolated according to secretion of IL-5 using the IL-5 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 266 IL-5-secreting CD4 + cells were enriched per 10 6 CD4 + T cells by using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, one IL-5-secreting CD4 + T cell was detected per 10 6 CD4 + T cells after enrichment. | IL-5 Secretion Assay – Cell Enrichment and Detection Kit (PE), humanFigure 1Human PBMCs were stimulated for 1 hour with CytoStim or left untreated. The responding cells were stained and isolated according to secretion of IL-5 using the IL-5 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 266 IL-5-secreting CD4 + cells were enriched per 10 6 CD4 + T cells by using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, one IL-5-secreting CD4 + T cell was detected per 10 6 CD4 + T cells after enrichment. |
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