The IL-4 Secretion Assay was developed for the sensitive detection of human IL-4-secreting cells.

Data and images for IL-4 Secretion Assay – Detection Kit (PE), human

Figures

Figure 1

PBMCs were stimulated with 1 µg/mL SEB for 16 hours. The responding cells were stained according to secretion of IL-4 using the IL-4 Secretion Assay - Detection Kit. In the stimulated sample, 0.307% of all CD4
+
T cells secreted IL-4. In the unstimulated control sample, 0.004% of all CD4
+
T cells secreted IL-4.
Stimulated sample
Unstimulated control
View details

Figure 1

PBMCs were stimulated with 1 µg/mL SEB for 16 hours. The responding cells were stained according to secretion of IL-4 using the IL-4 Secretion Assay - Detection Kit. In the stimulated sample, 0.307% of all CD4
+
T cells secreted IL-4. In the unstimulated control sample, 0.004% of all CD4
+
T cells secreted IL-4.
View details

Figure 1

PBMCs were stimulated with 1 µg/mL SEB for 16 hours. The responding cells were stained according to secretion of IL-4 using the IL-4 Secretion Assay - Detection Kit. In the stimulated sample, 0.307% of all CD4
+
T cells secreted IL-4. In the unstimulated control sample, 0.004% of all CD4
+
T cells secreted IL-4.

Specifications for IL-4 Secretion Assay – Detection Kit (PE), human

Overview

The IL-4 Secretion Assay was developed for the sensitive detection of human IL-4-secreting cells.

Detailed product information

Background information

Interleukin-4 (IL-4) is predominantly secreted by activated CD4
+
memory and effector T
H
2 cells, basophils, and mast cells. It induces and supports humoral immune responses for the neutralization of extracellular pathogens. The T
H
2 types of immune mechanisms can also be involved in immunologic disorders, such as IgE-mediated allergy. The IL-4 Secretion Assay (PE) can also be used for two-color cytokine analysis in combination with the IFN-γ Secretion Assay - Detection Kit (FITC) or the IFN-γ, IL-2, or IL-10 Secretion Assay - Detection Kits (APC).

Downstream applications

IL-4 Secretion Assays were used to isolate and analyze Tetanus Toxoid-specific
1
, allergen-specific
2,5
, and autoreactive
7
T cells from PBMCs, as well as tumor-specific cells from T cell lines
3
.
The IL-4 Secretion Assay was also used for isolation of IL-4-secreting human NK cells
4
, and for detection of IL-4-secreting NKT cells
6
.
IL-4-secreting CD8
+
T cells were isolated in combination with CD8 MultiSort MicroBeads.
8

References for IL-4 Secretion Assay – Detection Kit (PE), human

Publications

  1. Deepe, G. S. et al. (2009) The CCL7-CCL2-CCR2 axis regulates IL-4 production in lungs and fungal immunity. J. Immunol. 183(3): 1964-1974
  2. Brosterhus, H. et al. (1999) Enrichment and detection of live antigen-specific CD4(+) and CD8(+) T cells based on cytokine secretion. Eur. J. Immunol. 29: 4053-4059
  3. Akdis et al. (2000) MACS&more 4: 9-10
  4. Oelke, M. et al. (2000)
    Functional characterization of CD8
    +
    antigen-specific cytotoxic T lymphocytes after enrichment based on cytokine secretion: comparison with the MHC-tetramer technology.
    Scand. J. Immunol. 52: 544-549
  5. Deniz, G. et al. (2002) Human NK1 and NK2 subsets determined by purification of IFN-gamma-secreting and IFN-gamma-nonsecreting NK cells. Eur. J. Immunol. 32: 879-884
  6. Akdis, M. et al. (2004) Immune responses in healthy and allergic individuals are characterized by a fine balance between allergen-specific T regulatory 1 and T helper 2 cells. J. Exp. Med. 199: 1567-1575
  7. Araki, M. et al. (2002)
    Tʜ2 bias of CD4
    +
    NKT cells derived from multiple sclerosis in remission.
    Int. Immunol. 15: 279-288
  8. Veldman, C. et al. (2003) Dichotomy of autoreactive Th1 and Th2 cell responses to desmoglein 3 in patients with pemphigus vulgaris (PV) and healthy carriers of PV-associated HLA class II alleles. J. Immunol. 170: 635-642
  9. Schwaiger, S. et al. (2003)
    IL-4-producing CD8
    +
    T cells with a CD62L
    ++(bright)
    phenotype accumulate in a subgroup of older adults and are associated with the maintenance of intact humoral immunity in old age.
    J. Immunol. 170: 613-619

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