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Human peripheral blood mononuclear cells (PBMCs) were either left unstimulated (left image) or stimulated with 20 ng/mL phorbol 12-myristate 13-acetate (PMA) and 1 µg/mL ionomycin for 2 hours, followed by an incubation with 1 µg/mL brefeldin A for 4 hours. Cells were fixed, permeabilized, and directly stained with Anti-IL-3 antibodies as well as with CD154 antibodies. Flow cytometry was performed using the MACSQuant® Analyzer. CD4+ cells were pregated for the analysis. Cell debris were excluded from the analysis based on scatter signals.
Clone | BVD3-1F9 |
---|---|
Clonality | monoclonal |
Isotype | rat IgG1κ |
Isotype control | Isotype Control Antibody, rat IgG1 |
Host | rat |
Type of antibody | Primary antibodies |
Species | human |
Antigen | IL-3 |
Alternative names of antigen | MCGF, MULTI-CSF |
Molecular mass of antigen [kDa] | 15 |
Distribution of antigen | T cells |
Entrez Gene ID | 3562 |
RRID | AB_2652443, AB_2652444 |
Clone | BVD3-1F9 |
---|---|
Clonality | monoclonal |
Isotype | rat IgG1κ |
Isotype control | Isotype Control Antibody, rat IgG1 |
Host | rat |
Type of antibody | Primary antibodies |
Species | human |
Antigen | IL-3 |
Alternative names of antigen | MCGF, MULTI-CSF |
Molecular mass of antigen [kDa] | 15 |
Distribution of antigen | T cells |
Entrez Gene ID | 3562 |
RRID | AB_2652443, AB_2652444 |
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