The IL-17 Secretion Assay was developed for the detection of viable human IL-17–secreting leukocytes from PBMCs.

Data and images for IL-17 Secretion Assay – Detection Kits, human

Figures

Figure 1

PBMCs were stimulated with CytoStim for 4 hours or left untreated. Both samples were treated with a CD40 antibody to allow the surface detection of CD154. Subsequently, stimulated and unstimulated cells were analyzed by using the IL-17 Secretion Assay – Detection Kit (PE) or (APC). Secretion of IL-17 by CD154
+
cells is demonstrated. Data were gated on viable CD4
+
cells.
IL-17 Secretion Assay - Detection Kit (PE)
A:
B:
Restimulated sample
Unstimulated control
View details

Figure 1

PBMCs were stimulated with CytoStim for 4 hours or left untreated. Both samples were treated with a CD40 antibody to allow the surface detection of CD154. Subsequently, stimulated and unstimulated cells were analyzed by using the IL-17 Secretion Assay – Detection Kit (PE) or (APC). Secretion of IL-17 by CD154
+
cells is demonstrated. Data were gated on viable CD4
+
cells.
View details

Figure 1

PBMCs were stimulated with CytoStim for 4 hours or left untreated. Both samples were treated with a CD40 antibody to allow the surface detection of CD154. Subsequently, stimulated and unstimulated cells were analyzed by using the IL-17 Secretion Assay – Detection Kit (PE) or (APC). Secretion of IL-17 by CD154
+
cells is demonstrated. Data were gated on viable CD4
+
cells.
IL-17 Secretion Assay - Detection Kit (APC)
A:
B:
Restimulated sample
Unstimulated control
View details

Figure 1

PBMCs were stimulated with CytoStim for 4 hours or left untreated. Both samples were treated with a CD40 antibody to allow the surface detection of CD154. Subsequently, stimulated and unstimulated cells were analyzed by using the IL-17 Secretion Assay – Detection Kit (PE) or (APC). Secretion of IL-17 by CD154
+
cells is demonstrated. Data were gated on viable CD4
+
cells.
View details

Figure 1

PBMCs were stimulated with CytoStim for 4 hours or left untreated. Both samples were treated with a CD40 antibody to allow the surface detection of CD154. Subsequently, stimulated and unstimulated cells were analyzed by using the IL-17 Secretion Assay – Detection Kit (PE) or (APC). Secretion of IL-17 by CD154
+
cells is demonstrated. Data were gated on viable CD4
+
cells.

Specifications for IL-17 Secretion Assay – Detection Kits, human

Overview

The IL-17 Secretion Assay was developed for the detection of viable human IL-17–secreting leukocytes from PBMCs.

Detailed product information

Background information

IL-17 is produced by CD4
+
T helper cells, a T cell subset termed Th17, which also secrete IL-17F and IL-22 and express the NK cell marker CD161
1
. IL-17 secretion has also been described for other cell types, such as CD8
+
memory T cells.
Th17 cells are involved in the recruitment of neutrophils to control early stages of infections by a number of pathogens, such as extracellular bacteria and fungi. IL-17 and Th17 cells play an important role in immune-mediated inflammatory diseases, such as rheumatoid arthritis, psoriasis, multiple sclerosis, asthma, and inflammatory bowel disease.

References for IL-17 Secretion Assay – Detection Kits, human

Publications

  1. Dolcino, M. et al. (2018) Gene expression profiling in Behcet's disease indicates an autoimmune component in the pathogenesis of the disease and opens new avenues for targeted therapy. J Immunol Res
  2. Dolcino, M. et al. (2018) Immune response to rotavirus and gluten sensitivity. J Immunol Res
  3. Cosmi, L. et al. (2008)
    Human interleukin 17–producing cells originate from a CD161
    +
    CD4
    +
    T cell precursor.
    J. Exp. Med. 205: 1903-1916

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