The IL-13 Secretion Assay - Cell Enrichment and Detection Kit was developed for the sensitive detection as well as the enrichment of human IL-13-secreting cells.

Data and images for IL-13 Secretion Assay – Cell Enrichment and Detection Kit (PE), human

Figures

Figure 1

PBMCs were stimulated with CytoStim for 4 hours or left untreated. Both samples were treated with a CD40 antibody to allow the surface detection of CD154. Subsequently, IL-13-secreting cells were enriched and analyzed for IL-13 secretion and expression of CD154 using the IL-13 Secretion Assay - Cell Enrichment and Detection Kit, and CD154-APC. The plots show stimulated and unstimulated cells before and after separation. CD154 expression and IL-13 secretion by viable CD4
+
T cells is shown.
Stimulated sample
Before enrichment
After enrichment
View details

Figure 1

PBMCs were stimulated with CytoStim for 4 hours or left untreated. Both samples were treated with a CD40 antibody to allow the surface detection of CD154. Subsequently, IL-13-secreting cells were enriched and analyzed for IL-13 secretion and expression of CD154 using the IL-13 Secretion Assay - Cell Enrichment and Detection Kit, and CD154-APC. The plots show stimulated and unstimulated cells before and after separation. CD154 expression and IL-13 secretion by viable CD4
+
T cells is shown.
View details

Figure 1

PBMCs were stimulated with CytoStim for 4 hours or left untreated. Both samples were treated with a CD40 antibody to allow the surface detection of CD154. Subsequently, IL-13-secreting cells were enriched and analyzed for IL-13 secretion and expression of CD154 using the IL-13 Secretion Assay - Cell Enrichment and Detection Kit, and CD154-APC. The plots show stimulated and unstimulated cells before and after separation. CD154 expression and IL-13 secretion by viable CD4
+
T cells is shown.
Unstimulated control
Before enrichment
After enrichment
View details

Figure 1

PBMCs were stimulated with CytoStim for 4 hours or left untreated. Both samples were treated with a CD40 antibody to allow the surface detection of CD154. Subsequently, IL-13-secreting cells were enriched and analyzed for IL-13 secretion and expression of CD154 using the IL-13 Secretion Assay - Cell Enrichment and Detection Kit, and CD154-APC. The plots show stimulated and unstimulated cells before and after separation. CD154 expression and IL-13 secretion by viable CD4
+
T cells is shown.
View details

Figure 1

PBMCs were stimulated with CytoStim for 4 hours or left untreated. Both samples were treated with a CD40 antibody to allow the surface detection of CD154. Subsequently, IL-13-secreting cells were enriched and analyzed for IL-13 secretion and expression of CD154 using the IL-13 Secretion Assay - Cell Enrichment and Detection Kit, and CD154-APC. The plots show stimulated and unstimulated cells before and after separation. CD154 expression and IL-13 secretion by viable CD4
+
T cells is shown.

Specifications for IL-13 Secretion Assay – Cell Enrichment and Detection Kit (PE), human

Overview

The IL-13 Secretion Assay - Cell Enrichment and Detection Kit was developed for the sensitive detection as well as the enrichment of human IL-13-secreting cells.

Detailed product information

Background information

Interleukin-13 (IL-13) is produced primarily by activated Th2 cells, but also by mast cells, NK cells, and dendritic cells. IL-13 exerts anti-inflammatory effects on monocytes and macrophages. Furthermore, it inhibits the expression of pro-inflammatory cytokines, such as IL-1β, TNF-α, IL-6, and IL-8. IL-13 has also been shown to enhance B cell proliferation and to induce isotype switching, resulting in an increased production of IgE.
IL-13, in contrast to IL-4, fails to induce Th2 cell differentiation, one of the hallmarks of the allergic response.

Columns

MS, LS, or autoMACS
®
Columns.

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