The IL-12 Secretion Assay - Cell Enrichment and Detection Kit was developed for the sensitive detection and as well as the enrichment of human cells secreting the p70 subunit of IL-12.

Data and images for IL-12 Secretion Assay – Cell Enrichment and Detection Kit (PE), human

Figures

Figure 1

Human PBMCs depleted of CD14
+
cells were stimulated for 4 hours with LPS (10 ng/ml) and for 2 hours with R-848 (2.5 μg/ml), or left untreated. The stimulated and unstimulated cells were stained and isolated according to the secretion of IL-12 using the IL-12 Secretion Assay - Cell Enrichment and Detection Kit. Additionally, cells were stained with CD11c-APC.
Stimulated sample
Before enrichment
After enrichment
View details

Figure 1

Human PBMCs depleted of CD14
+
cells were stimulated for 4 hours with LPS (10 ng/ml) and for 2 hours with R-848 (2.5 μg/ml), or left untreated. The stimulated and unstimulated cells were stained and isolated according to the secretion of IL-12 using the IL-12 Secretion Assay - Cell Enrichment and Detection Kit. Additionally, cells were stained with CD11c-APC.
View details

Figure 1

Human PBMCs depleted of CD14
+
cells were stimulated for 4 hours with LPS (10 ng/ml) and for 2 hours with R-848 (2.5 μg/ml), or left untreated. The stimulated and unstimulated cells were stained and isolated according to the secretion of IL-12 using the IL-12 Secretion Assay - Cell Enrichment and Detection Kit. Additionally, cells were stained with CD11c-APC.
Unstimulated control
Before enrichment
After enrichment
View details

Figure 1

Human PBMCs depleted of CD14
+
cells were stimulated for 4 hours with LPS (10 ng/ml) and for 2 hours with R-848 (2.5 μg/ml), or left untreated. The stimulated and unstimulated cells were stained and isolated according to the secretion of IL-12 using the IL-12 Secretion Assay - Cell Enrichment and Detection Kit. Additionally, cells were stained with CD11c-APC.
View details

Figure 1

Human PBMCs depleted of CD14
+
cells were stimulated for 4 hours with LPS (10 ng/ml) and for 2 hours with R-848 (2.5 μg/ml), or left untreated. The stimulated and unstimulated cells were stained and isolated according to the secretion of IL-12 using the IL-12 Secretion Assay - Cell Enrichment and Detection Kit. Additionally, cells were stained with CD11c-APC.

Specifications for IL-12 Secretion Assay – Cell Enrichment and Detection Kit (PE), human

Overview

The IL-12 Secretion Assay - Cell Enrichment and Detection Kit was developed for the sensitive detection and as well as the enrichment of human cells secreting the p70 subunit of IL-12.

Detailed product information

Background information

Interleukin-12 (IL-12) is a 70-75 kDa heterodimeric cytokine composed of a 40 kDa heavy chain and a 35 kDa light chain. It is mainly produced by monocytes and macrophages, as well as dendritic cells primarily in response to stimulation with bacterial or viral antigens. IL-12 displays various effects on both NK and T cells, playing an essential role in the interaction between innate and adaptive immunity.

Columns

MS, LS, or autoMACS
®
Columns.

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