Clone:
REA842
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
ICFC
Alternative names:
IL10, CSIF, GVHDS, TGIF, IL10A, Interleukin-10

Extended validation for IL-10 Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA842
2050B-
JES3-12G8-
JES3-19F1++
JES3-9D7++
Cells were incubated with an excess of purified unconjugated IL-10 (REA842) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for IL-10. Human peripheral blood mononuclear cells (PBMCs) were stimulated with CytoStim for 1 hour, followed by an incubation with 1µg/mL brefeldin A for 5 hours. Cells were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized followed by a staining with IL-10 antibodies. As a control, IL-10 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. CD4+ cells were pregated for the analysis. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for IL-10. Human peripheral blood mononuclear cells (PBMCs) were stimulated with CytoStim for 1 hour, followed by an incubation with 1µg/mL brefeldin A for 5 hours. Cells were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized followed by a staining with IL-10 antibodies. As a control, IL-10 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. CD4+ cells were pregated for the analysis. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for IL-10. Human peripheral blood mononuclear cells (PBMCs) were stimulated with CytoStim for 1 hour, followed by an incubation with 1µg/mL brefeldin A for 5 hours. Cells were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized followed by a staining with IL-10 antibodies. As a control, IL-10 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. CD4+ cells were pregated for the analysis. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for IL-10. Human peripheral blood mononuclear cells (PBMCs) were stimulated with CytoStim for 1 hour, followed by an incubation with 1µg/mL brefeldin A for 5 hours. Cells were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized followed by a staining with IL-10 antibodies. As a control, IL-10 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. CD4+ cells were pregated for the analysis. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for IL-10. Human peripheral blood mononuclear cells (PBMCs) were stimulated with CytoStim for 1 hour, followed by an incubation with 1µg/mL brefeldin A for 5 hours. Cells were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized followed by a staining with IL-10 antibodies. As a control, IL-10 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. CD4+ cells were pregated for the analysis. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for IL-10. Human peripheral blood mononuclear cells (PBMCs) were stimulated with CytoStim for 1 hour, followed by an incubation with 1µg/mL brefeldin A for 5 hours. Cells were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized followed by a staining with IL-10 antibodies. As a control, IL-10 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. CD4+ cells were pregated for the analysis. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Specifications for IL-10 Antibody, anti-human, REAfinity™

Overview

Clone REA842 recognizes the interleukin 10 (IL-10). IL-10 is a cytokine predominantly secreted by CD4
+
memory and effector T cells and antigen-presenting cells, for example, monocytes/macrophages and dendritic cells. IL-10 has important suppressive functions on immune responses and is believed to be involved in the maintenance of tolerance. IL-10 blocks activation of cytokine synthesis by Tʜ1 cells, activated monocytes, and NK cells. It can stimulate immunoglobulin production by B cells.
Additional information: Clone REA842 displays negligible binding to Fc receptors.

Alternative names

IL10, CSIF, GVHDS, TGIF, IL10A, Interleukin-10

Detailed product information

Technical specifications

CloneREA842
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (I), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, non-human primate
Cross-reactivity
rhesus monkey (
Macaca mulatta
)
AntigenIL-10
Alternative names of antigenIL10, CSIF, GVHDS, TGIF, IL10A, Interleukin-10
Molecular mass of antigen [kDa]19
Distribution of antigenT cells, monocytes, macrophages, dendritic cells
Entrez Gene ID3586
RRIDAB_2652317, AB_2652318, AB_2652319, AB_2652320

References for IL-10 Antibody, anti-human, REAfinity™

Publications

  1. Gesser, B. et al. (1997) Identification of functional domains on human interleukin 10. Proc. Natl. Acad. Sci. U.S.A. 94(26): 14620-14625
  2. Ng, T. H. et al. (2013) Regulation of adaptive immunity; the role of interleukin-10. Front Immunol 129

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