Alternative names:
OSM

Data and images for Human Oncostatin M IS

Figures

Figure 1

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Human Oncostatin M IS activity assay.
The biological activity of Human Oncostatin M IS, premium grade, was determined by prolifertaion of TF-1 cells.

Figure 1

Human Oncostatin M IS activity assay.
The biological activity of Human Oncostatin M IS, premium grade, was determined by prolifertaion of TF-1 cells.

Figure 2

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Human Oncostatin M IS biological activity.
Activity of Human Oncostatin M IS, premium grade (red bar) was compared to another commercially available product (black bar).

Figure 2

Human Oncostatin M IS biological activity.
Activity of Human Oncostatin M IS, premium grade (red bar) was compared to another commercially available product (black bar).

Figure 3

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SDS-PAGE of Human Oncostatin M IS, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 3

SDS-PAGE of Human Oncostatin M IS, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 4

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Mass spectrometry analysis (ESI-MS) of Human Oncostatin M IS, premium grade. The peak corresponds to the calculated molecular mass of 22044 Da.

Figure 4

Mass spectrometry analysis (ESI-MS) of Human Oncostatin M IS, premium grade. The peak corresponds to the calculated molecular mass of 22044 Da.

Specifications for Human Oncostatin M IS

Overview

Human Oncostatin M IS is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays. IS stands for "improved sequence" and is a variant of Human Oncostatin M with a proprietary amino acid substitution.

Applications

Human Oncostatin M IS can be used for a variety of applications including:
  • Study of hematopoiesis, osteogenesis, and neurogenesis.
  • Expansion of human fibroblasts.

Alternative names

OSM

Detailed product information

Background information

Oncostatin M, also known as OSM, is a member of the interleukin 6 (IL-6) related cytokine subfamily that includes IL-6, interleukin 11 (IL-11), leukemia inhibitory factor (LIF), and granulocyte colony-stimulating factor (G-CSF). It is a growth and differentiation factor expressed by activated T cells and monocytes that is involved in hematopoiesis, osteogenesis, and neurogenesis. Oncostatin M induces the expression of cytokines like G-CSF and GM-CSF by endothelial cells. It inhibits the growth of a number of tumor cell lines and promotes the growth of human fibroblasts and smooth muscle cells. Human Oncostatin M is active on murine cells.

Biological activity

  • Proliferation assay using TF-1 cells (NIBSC 93/564)
  • premium grade: ≥ 2×
    10
    7
    U/mg
  • research grade: ≥ 8×
    10
    6
    U/mg

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Resources for Human Oncostatin M IS

Documents and Protocols

Background information

Premium-grade cytokine benefits

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for Human Oncostatin M IS

Publications

  1. Kitamura, T. et al. (1989) Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J. Cell. Physiol. 140: 323-334