Human LIF stands for leukemia inhibitory factor. Human LIF is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Data and images for Human LIF

Figures

Figure 1

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Human LIF activity assay.
The biological activity of Human LIF, premium grade was determined by an inhibition assay using mouse M1 cells.

Figure 1

Human LIF activity assay.
The biological activity of Human LIF, premium grade was determined by an inhibition assay using mouse M1 cells.

Figure 2

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Purity of Human LIF, premium grade was analyzed under reducing (R) and non-reducing (NR) conditions.

Figure 2

Purity of Human LIF, premium grade was analyzed under reducing (R) and non-reducing (NR) conditions.

Figure 3

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Mass spectrometry analysis (ESI-MS) of Human LIF, premium grade. The peak corresponds to the calculated molecular mass of 19710 Da.

Figure 3

Mass spectrometry analysis (ESI-MS) of Human LIF, premium grade. The peak corresponds to the calculated molecular mass of 19710 Da.

Specifications for Human LIF

Overview

Human LIF stands for leukemia inhibitory factor. Human LIF is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human LIF may be used for a variety of applications, including:
  • Study of reprogramming and maintenance of human iPS cells to a naive-like stage.
  • Investigation of hematopoiesis, bone and neural development.

Detailed product information

Background information

Leukemia inhibitory factor (LIF) is a pleiotropic cytokine, which is critically involved in embryonic development and blastocyst implantation. LIF belongs to the interleukin 6 family and functions through the gp130 activation of STAT3. It is a key factor that prevents embryonic stem cells (ESC) from differentiating, and is therefore used for maintenance of ESC and iPSC cultures. LIF has a role in the reprogramming and mantainance of ES cells to a naive-like stage. Additionally, LIF affects hematopoiesis, neural development, bone and energy metabolism, and inflammation.

Biological activity

  • Inhibition assay using mouse M1 cells (NIBSC 93/562).
  • research grade: ≥ 4×
    10
    6
    U/mg
  • premium grade: ≥ 8×
    10
    6
    U/mg

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Resources for Human LIF

Documents and Protocols

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for Human LIF

Publications

  1. Rose, T. M. and Bruce, A. G. (1991) Oncostatin M is a member of a cytokine family that includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6. Proc. Natl. Acad. Sci. U.S.A. 88: 8641-8645
  2. Barral, S. et al. (2013)
    Efficient neuronal
    in vitro
    and
    in vivo
    differentiation after immunomagnetic purification of mESC derived neuronal precursors.
    Stem Cell Res. 10(2): 133-46

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