Recombinant human Flt3-Ligand can be used to generate and expand hematopoietic stem cells or to differentiate the hematopoietic progenitors. In the myeloid lineage this cytokine is crucial for dendritic cells and in the lymphoid lineage it its involved in B and NK cell differentiation. Human Flt3-Ligand recombinant protein is developed for use in cell culture, differentiation studies, and functional assays.

Data and images for Human Flt3-Ligand

Figures

Figure 1

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Human Flt3-Ligand activity assay.
The biological activity of Human Flt3-Ligand is determined by proliferation assay using OCI-AML5 cells. Activity of Human Flt3-Ligand, premium grade, (red line) was compared to another commercially available product (black line).

Figure 1

Human Flt3-Ligand activity assay.
The biological activity of Human Flt3-Ligand is determined by proliferation assay using OCI-AML5 cells. Activity of Human Flt3-Ligand, premium grade, (red line) was compared to another commercially available product (black line).

Figure 2

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SDS-PAGE of Human Flt3-Ligand, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 2

SDS-PAGE of Human Flt3-Ligand, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 3

View details
Human Flt3-Ligand biological activity.
Activity of Human Flt3-Ligand, premium grade, (red bar) was compared to another commercially available product (black bar).

Figure 3

Human Flt3-Ligand biological activity.
Activity of Human Flt3-Ligand, premium grade, (red bar) was compared to another commercially available product (black bar).

Figure 4

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Mass spectrometry analysis (ESI-MS) of Human Flt3-Ligand, premium grade. The peak corresponds to the calculated molecular mass of 17934 Da.

Figure 4

Mass spectrometry analysis (ESI-MS) of Human Flt3-Ligand, premium grade. The peak corresponds to the calculated molecular mass of 17934 Da.

Specifications for Human Flt3-Ligand

Overview

Recombinant human Flt3-Ligand can be used to generate and expand hematopoietic stem cells or to differentiate the hematopoietic progenitors. In the myeloid lineage this cytokine is crucial for dendritic cells and in the lymphoid lineage it its involved in B and NK cell differentiation. Human Flt3-Ligand recombinant protein is developed for use in cell culture, differentiation studies, and functional assays.

Applications

Human Flt3-Ligand can be used for a variety of applications, including:
  • In vitro expansion of CD34+ hematopoietic progenitor cells.
  • Differentiation of ES-derived cells towards the hematopoietic lineage.
  • In vitro generation of Langerhans cells, dendritic cells, or eosinophils from CD34+ cells.

Detailed product information

Background information

Fms-related tyrosin kinase 3 ligand (Flt3-Ligand) is a growth factor that regulates early hematopoiesis. Flt3-Ligand belongs to a small family of α-helical cytokines and promotes in synergy with other growth factors like G-CSF, GM-CSF, SCF, and IL-3 the proliferation and differentiation of primitive hematopoietic stem cells. Early B cell lineage differentiation as well as expansion of monocytes and immature dendritic cells is stimulated. Flt3-Ligand is expressed by T lymphocytes and bone marrow stromal fibroblasts as a membrane-bound and a soluble isoform. Both isoforms signal through the tyrosine kinase receptor Flt3/Flk-2, which is restricted to cells of hematopoietic origin. Human Flt3-Ligand is also active on mouse cells.

Biological activity

  • Proliferation of OCI-AML5 cells (NIBSC 96/532)
  • premium grade: ≥ 5×
    10
    5
    U/mg
  • research grade: ≥ 2×
    10
    5
    U/mg

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Resources for Human Flt3-Ligand

Documents and Protocols

Background information

Premium-grade cytokine benefits

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for Human Flt3-Ligand

Publications

  1. Dussiau, C. et al. (2015) Targeting IRAK1 in T-cell acute lymphoblastic leukemia. Oncotarget. 6: 18956-18965
  2. Laurenti, E. et al. (2015) CDK6 levels regulate quiescence exit in human hematopoietic stem cells. Cell Stem Cell 16(3): 302-313
  3. Brault, J. et al. (2014)
    Optimized generation of functional neutrophils and macrophages from patient-specific induced pluripotent stem cells:
    ex vivo
    models of X
    0
    -linked, AR22
    0
    - and AR47
    0
    - chronic granulomatous diseases.
    Biores Open Access 3(6): 311-326
  4. Velardi, E. et al. (2014) Sex steroid blockade enhances thymopoiesis by modulating Notch signaling. J. Exp. Med. 211(12): 2341-2349
  5. Narla, A. et al. (2011) Dexamethasone and lenalidomide have distinct functional effects on erythropoiesis. Blood 118(8): 2296-2304
  6. Meyer, C. and Drexler, H.G. (1999) FLT3 ligand inhibits apoptosis and promotes survival of myeloid leukemia cell lines. Leuk. Lymphoma 32: 577-581

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