FGF-8b stands for fibroblast growth factor 8b. Human FGF-8b is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Data and images for Human FGF-8b

Figures

Figure 1

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Human FGF-8b activity assay.
The biological activity of Human FGF-8b is determined by proliferation assay in the presence of sodium heparin using 3T3 cells.

Figure 1

Human FGF-8b activity assay.
The biological activity of Human FGF-8b is determined by proliferation assay in the presence of sodium heparin using 3T3 cells.

Figure 2

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Human FGF-8b biological activity.
Activity of Human FGF-8b, premium grade (red bar) was compared to another commercially available product (black bar).

Figure 2

Human FGF-8b biological activity.
Activity of Human FGF-8b, premium grade (red bar) was compared to another commercially available product (black bar).

Figure 3

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SDS-PAGE of Human FGF-8b, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 3

SDS-PAGE of Human FGF-8b, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 4

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Mass spectrometry analysis (ESI-MS) of Human FGF-8b, premium grade. The peak corresponds to the calculated molecular mass of 22522 Da.

Figure 4

Mass spectrometry analysis (ESI-MS) of Human FGF-8b, premium grade. The peak corresponds to the calculated molecular mass of 22522 Da.

Specifications for Human FGF-8b

Overview

FGF-8b stands for fibroblast growth factor 8b. Human FGF-8b is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human FGF-8b can be used for a variety of applications, including:
  • Differentiation of embryonic stem cells into neutral progenitor cells.
  • Differentiation of embryonic stem cell-derived neural progenitor cells into dopaminergic neurons.
  • Survival of neural precursor cells and differentiation into astroglial cells.
  • Stimulation of osteoblast proliferation.
  • Proliferation of endothelial cells.
  • Proliferation of myogenic cells.
  • Tumor growth studies of prostate and breast cancer.
  • Protection of hippocampal cultures from oxidative stress.

Detailed product information

Background information

Fibroblast growth factor 8 (FGF-8), originally identified as androgen-induced growth factor, is a heparin-binding protein, which has mitogenic and transforming activity. Eight secreted FGF-8 isoforms (FGF-8a to h) have been identified in mice, whereas four have been described in humans (a, b, e, and f). FGF-8b appears to be the predominant form and has been shown to have the most important oncogenic transforming capacity. FGF-8 exerts a pivotal role in embryogenesis. It is expressed during gastrulation and influences brain, limb, heart and facial development in the mouse embryo. In addition, FGF-8 was shown to stimulate osteoblast proliferation. The amino acid sequence of human FGF8-b shares 100% identity with mouse FGF-8b.

Biological activity

  • Proliferation of 3T3 cells
  • premium grade: ≥ 2×
    10
    4
    U/mg
  • research grade: ≥ 1×
    10
    4
    U/mg

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Resources for Human FGF-8b

Documents and Protocols

Background information

Premium-grade cytokine benefits

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.