EGF stands for epidermal growth factor. Human EGF is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Data and images for Human EGF

Figures

Figure 1

View details
In vitro generation of primary mouse neurospheres.
A single-cell suspension was prepared from mouse brain using the Neural Tissue Dissociation Kit (P) and the gentleMACS™ Dissociator. Isolated Prominin-1
+
cells were cultivated for 7 days in MACS NeuroMedium containing MACS NeuroBrew, Human FGF-2 (20 ng/mL), and Human EGF (20 ng/mL).

Figure 1

In vitro generation of primary mouse neurospheres.
A single-cell suspension was prepared from mouse brain using the Neural Tissue Dissociation Kit (P) and the gentleMACS™ Dissociator. Isolated Prominin-1
+
cells were cultivated for 7 days in MACS NeuroMedium containing MACS NeuroBrew, Human FGF-2 (20 ng/mL), and Human EGF (20 ng/mL).

Figure 2

View details
Human EGF activity assay.
The biological activity of Human EGF is determined by proliferation assay using 3T3 cells. Activity of Human EGF, premium grade, (red line) was compared to another commercially available product (black line).

Figure 2

Human EGF activity assay.
The biological activity of Human EGF is determined by proliferation assay using 3T3 cells. Activity of Human EGF, premium grade, (red line) was compared to another commercially available product (black line).

Figure 3

View details
SDS-PAGE of Human EGF, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 3

SDS-PAGE of Human EGF, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 4

View details
Mass spectrometry analysis (ESI-MS) of Human EGF, premium grade. The peak corresponds to the calculated molecular mass of 6347 Da.

Figure 4

Mass spectrometry analysis (ESI-MS) of Human EGF, premium grade. The peak corresponds to the calculated molecular mass of 6347 Da.

Specifications for Human EGF

Overview

EGF stands for epidermal growth factor. Human EGF is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human EGF can be used for a variety of applications, including:
  • Proliferation and differentiation of a wide variety of cell types deriving from ectoderm and mesoderm.

Detailed product information

Background information

Epidermal growth factor (EGF) is the prototype of the large family of EGF-like proteins with a common structural motif comprising three intramolecular disulfide bonds. EGF is produced by various cell types like mammary gland cells, gut epithelial cells, and cells in the nervous system and the kidney. Production of EGF is induced by testosterone and inhibited by estrogens. EGF stimulates the proliferation and differentiation of mesenchymal cells, acts as a mitogen for fibroblasts, epithelial and endothelial cells, and promotes colony formation of epidermal cells.

Biological activity

  • Proliferation of 3T3 cells
  • premium grade: ≥ 0.8×
    10
    6
    IU/mg
    (typical activity: ≥ 2×
    10
    6
    IU/mg
    )
  • research grade: ≥ 0.5×
    10
    6
    IU/mg

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Resources for Human EGF

Documents and Protocols

Background information

Premium-grade cytokine benefits

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for Human EGF

Publications

  1. Diaz-Guerra E. et al. (2012) Intrinsic cues and hormones control mouse mammary epithelial tree size. FASEB J. 26(9): 3844-3853
  2. Robinson, C. J. and Gaines-Das, R. (1996)
    The international standard for epidermal growth factor (EGF): comparison of candidate preparations by
    in vitro
    bioassays and immunoassays. National Institute for Biological Standards and Control.
    Growth Factors 13: 163-170

Related products for
Human EGF

1 products available

Seems like you are coming from USA!
Do you want to visit our website in your country?