Human CD40-Ligand is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Data and images for Human CD40-Ligand Multimer Kits

Figures

Figure 1

View details
Human CD40-Ligand activity assay:
The specific activity is determined by proliferation assay using enriched CD19
+
B cells in the presence of the cross-linking antibody and of 50 IU/mL Interleukin 4. Activity of Human CD40-Ligand, premium grade (red line) was compared to another commercially available product (black line).

Figure 1

Human CD40-Ligand activity assay:
The specific activity is determined by proliferation assay using enriched CD19
+
B cells in the presence of the cross-linking antibody and of 50 IU/mL Interleukin 4. Activity of Human CD40-Ligand, premium grade (red line) was compared to another commercially available product (black line).

Figure 2

View details
Human CD40-Ligand activity assay:
The specific activity is determined by
activation assay
using enriched CD19
+
B cells in the presence of the cross-linking antibody and of 50 IU/mL Interleukin 4. Activity of Human CD40-Ligand, premium grade (red line) was compared to another commercially available product (black line).

Figure 2

Human CD40-Ligand activity assay:
The specific activity is determined by
activation assay
using enriched CD19
+
B cells in the presence of the cross-linking antibody and of 50 IU/mL Interleukin 4. Activity of Human CD40-Ligand, premium grade (red line) was compared to another commercially available product (black line).

Specifications for Human CD40-Ligand Multimer Kits

Overview

Human CD40-Ligand is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human CD40-Ligand can be used for a variety of applications, including:
  • Stimulation of B cell activation and proliferation.
  • Stimulation of dendritic cell maturation.
  • Induction of cytokine production in peripheral blood monocytes and T cells.

Detailed product information

Background information

CD40-Ligand, also known as CD40L, CD154, TRAP, or TNFSF5 is a member of the TNF superfamily transiently expressed on the surface of activated CD4
+
T lymphocytes. It is either expressed membrane-bound or proteolytically released as a soluble form which comprises 2/3 of the extracellular domain. Its receptor CD40 is found on antigen-presenting cells such as B cells, dendritic cells, and macrophages. CD40L-CD40 interaction is important in T cell-APC (antigen-presenting cell) interaction and is e.g. involved in B cell differentiation and proliferation, isotype class-switching, and protection of B cells from apoptosis.
The CD40-Ligand Multimer Kit contains the CD40-Ligand and a cross-linking antibody for multimerization to increase the biological activity of CD40-Ligand.

Quality description

Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Resources for Human CD40-Ligand Multimer Kits

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

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