Human Activin A is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Data and images for Human Activin A

Figures

Figure 1

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Human Activin A activity assay.
The biological activity of Human Activin A, premium grade was determined by inhibition assay using MPC-11 cells.

Figure 1

Human Activin A activity assay.
The biological activity of Human Activin A, premium grade was determined by inhibition assay using MPC-11 cells.

Figure 2

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SDS-PAGE of Human Activin A, premium grade
under reducing (R) and non-reducing (NR) conditions.

Figure 2

SDS-PAGE of Human Activin A, premium grade
under reducing (R) and non-reducing (NR) conditions.

Figure 3

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Mass spectrometry analysis (ESI-MS) of Human Activin A, premium grade. The peak corresponds to the calculated molecular mass of 25934 Da.

Figure 3

Mass spectrometry analysis (ESI-MS) of Human Activin A, premium grade. The peak corresponds to the calculated molecular mass of 25934 Da.

Specifications for Human Activin A

Overview

Human Activin A is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human Activin A can be used for a variety of applications, including:
  • Maintenance of pluripotency of embryonic stem cells under feeder- and serum-free conditions.
  • Differentiation of embryonic stem cells and iPS cells.
  • Stimulation and differentiation of mesenchymal cells.

Detailed product information

Background information

Activin A is a member of the TGF-β superfamily and is involved in a wide range of biological processes like growth and differentiation of several tissues. It has been described to affect embryogenesis and hematopoiesis and is necessary for the maintenance of self-renewal and pluripotency of human embryonic stem cells (hESCs). It supports long-term feeder and serum-free growth of hESCs. Activin A induces the expression of Oct4, Nanog, Nodal, Wnt3, FGF-2, and FGF-8 and suppresses the BMP signal. It also controls the expression and secretion of hormones like follicle stimulating hormone (FSH), prolactin, and ACTH (corticotropin). The amino acid sequence of human, mouse, and rat activin A shares 100% identity.

Biological activity

  • Inhibition assay using MPC-11 cells (NIBSC 91/626)
  • premium grade: ≥ 0.6×
    10
    3
    U/mg
  • research grade: ≥ 0.4×
    10
    3
    U/mg

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Resources for Human Activin A

Documents and Protocols

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for Human Activin A

Publications

  1. Phillips, D. J. et al. (1999)
    A sensitive and specific
    in vitro
    bioassay for activin using a mouse plasmacytoma cell line, MPC-11.
    J. Endocrinol. 162(1): 111-116

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