Clone:
REA303
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
HLA-DQA1, HLA-DQA2, HLA-DQB1, HLA-DQB2

Extended validation for HLA-DQ Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA303
SK10-
HLADQ1-
Tü169++
SPVL3++
REA332-
Cells were incubated with an excess of purified unconjugated HLA-DQ (REA303) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for Anti-HLA-DQ. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-HLA-DQ antibodies and plotted against the side scatter. As a control, Anti-HLA-DQ antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-HLA-DQ. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-HLA-DQ antibodies and plotted against the side scatter. As a control, Anti-HLA-DQ antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-HLA-DQ. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-HLA-DQ antibodies and plotted against the side scatter. As a control, Anti-HLA-DQ antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-HLA-DQ. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-HLA-DQ antibodies and plotted against the side scatter. As a control, Anti-HLA-DQ antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-HLA-DQ. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-HLA-DQ antibodies and plotted against the side scatter. As a control, Anti-HLA-DQ antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for Anti-HLA-DQ. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-HLA-DQ antibodies and plotted against the side scatter. As a control, Anti-HLA-DQ antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using HLA-DQ (REA303). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using HLA-DQ (REA303). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using HLA-DQ (REA303). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for HLA-DQ Antibody, anti-human, REAfinity™

Overview

Clone REA303 recognizes HLA-DQ, an αβ heterodimer of the MHC class II type. Expressed on the surface of most nucleated cells, MHC class II molecules play an important role in the immune system. They are essential in the defense against infection and are a main consideration in transplantation medicine. In addition to presenting antigenic peptides from predominantly extracellular sources to CD4
+
T cells, MHC class II molecules also mediate the thymic selection of T helper cells. MHC class II molecules consist of an α and β chain and are transported to endosomal-lysosomal compartments by the invariant chain. In humans, MHC class II molecules are encoded by three different loci, HLA-DR, -DQ, and -DP, which display 70% similarity to each other. Both chains of HLA-DQ are polymorphic. Despite the essential function of MHC class II molecules in immune defense against pathogens, some alleles are frequently linked to immune diseases. Two autoimmune diseases in which HLA-DQ is involved are coeliac disease and diabetes mellitus type 1.
Additional information: Clone REA303 displays negligible binding to Fc receptor

Alternative names

HLA-DQA1, HLA-DQA2, HLA-DQB1, HLA-DQB2

Detailed product information

Technical specifications

CloneREA303
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenHLA-DQ
Alternative names of antigenHLA-DQA1, HLA-DQA2, HLA-DQB1, HLA-DQB2
Molecular mass of antigen [kDa]26(average of subunits)
Distribution of antigenB cells, dendritic cells, macrophages, monocytes
Entrez Gene ID3117, 3118, 3119, 3120, 3121
RRIDAB_2811632, AB_2857697, AB_2857685, AB_2652145, AB_2652146, AB_2652149, AB_2652150, AB_2652151, AB_2652152, AB_2652153, AB_2652154, AB_2811637

References for HLA-DQ Antibody, anti-human, REAfinity™

Publications

  1. van Lith, M. et al. (2010) HLA-DP, HLA-DQ, and HLA-DR have different requirements for invariant chain and HLA-DM. J. Biol. Chem. 285(52): 40800-40808
  2. Jones, E. Y. et al. (2006) MHC class II proteins and disease: a structural perspective. Nat. Rev. Immunol. 6(4): 271-282
  3. Klitz, W. et al. (2003) New HLA haplotype frequency reference standards: high-resolution and large sample typing of HLA DR-DQ haplotypes in a sample of European Americans. Tissue Antigens 62(4): 296-307

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