Type of antibody:
Primary antibodies, Recombinant antibodies
recombinant human IgG1
Alternative names:

Extended validation for HLA-B7, B27 Antibody, anti-human, REAfinity™


Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA176
Cells were incubated with an excess of purified unconjugated HLA-B7, B27 (REA176) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Specifications for HLA-B7, B27 Antibody, anti-human, REAfinity™


Clone REA176 recognizes HLA B7 and B27 class I human leukocyte antigens (HLA). Expressed on the surface of most nucleated cells, class I molecules are heterodimeric molecules and consist of a type I integral membrane α heavy chain and soluble β2 microglobulin protein. The extracellular region of the heavy chain further consists of three domains, of which one comprises the peptide binding groove. Antigens binding to class I molecules are 8–10 amino acids long and play an important role in recognition of the virus infected and malignant cells by cytotoxic T lymphocytes (CTLs). In addition, class I molecules interact with NK cell receptors to modulate the activity of NK cells. HLA-B7 is a risk factor for cervical cancer, sarcoidosis, and early-onset spondylarthropathies. HLA-B27 is known for its strong association with inflammatory spondyloarthropathies. It has been also discovered as a protective factor against some severe viral infections.
Additional information: Clone REA176 displays negligible binding to Fc receptors.

Alternative names


Detailed product information

Technical specifications

Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
AntigenHLA-B7, B27
Alternative names of antigenHLA-B, AS, SPDA1
Molecular mass of antigen [kDa]38
Distribution of antigenother
Entrez Gene ID3106
RRIDAB_2733749, AB_2752059, AB_2752046, AB_2811539, AB_2811531, AB_2652117, AB_2652118, AB_2922092, AB_2922059, AB_2922151, AB_2922115, AB_2733748

Resources for HLA-B7, B27 Antibody, anti-human, REAfinity™


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References for HLA-B7, B27 Antibody, anti-human, REAfinity™


  1. Akram, A. et al. (2014) HLA-B27, but not HLA-B7, immunodominance to influenza is ERAP dependent. J Immunol 192(12): 5520-5528
  2. Akram, A. et al. (2013) Co-expression of HLA-B7 and HLA-B27 alleles is associated with B7-restricted immunodominant responses following influenza infection. Eur. J. Immunol. 43(12): 3254-3267
  3. den Uyl, D. et al. (2004) Progression of HIV to AIDS: a protective role for HLA-B27? AIDS Rev. 6(2): 89-96

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