Clone:
REA379
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
ICFC, MICS, IF, IHC
Alternative names:
H3F3B, H3.3B, H3.3A, H3F3, H3F3A, H3 (pS28), H3.1, H3.2, H3.3

Specifications for Histone H3 pS28 Antibody, anti-human/mouse, REAfinity™

Overview

Clone REA379 recognizes the human and mouse histone H3 antigen phosphorylated at serine 28 (pS28). Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Phosphorylation of histone H3 at pS10 and pS28 is very important for chromosome condensation and segregation during mitosis. The phosphorylation of histone H3 at pS10 appears to be involved in the initiation of chromosome condensation but not the maintenance of it. Although phosphorylation of histone H3 at pS10 is related to chromosome assembly, congregation at the metaphase plate, and segregation, phosphorylation of histone H3 at pS28 occurs in chromosomes predominantly during early mitosis and coincides with the initiation of mitotic chromosome condensation in various mammalian cell lines.
Additional information: Clone REA379 displays negligible binding to Fc receptors.

Alternative names

H3F3B, H3.3B, H3.3A, H3F3, H3F3A, H3 (pS28), H3.1, H3.2, H3.3

Detailed product information

Technical specifications

CloneREA379
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (I), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, mouse, rat, other
Cross-reactivityDrosophila, rat, cow
AntigenHistone H3 pS28
Alternative names of antigenH3F3B, H3.3B, H3.3A, H3F3, H3F3A, H3 (pS28), H3.1, H3.2, H3.3
Molecular mass of antigen [kDa]15
Distribution of antigenother
Entrez Gene ID3020, 3021
RRIDAB_2651991, AB_2651992, AB_2651995

References for Histone H3 pS28 Antibody, anti-human/mouse, REAfinity™

Publications

  1. Wells, D. et al. (1985) Structure of a human histone cDNA: evidence that basally expressed histone genes have intervening sequences and encode polyadenylylated mRNAs. Proc. Natl. Acad. Sci. U.S.A. 82(9): 2834-2838
  2. Choi, H. S. et al. (2005) Phosphorylation of Ser28 in histone H3 mediated by mixed lineage kinase-like mitogen-activated protein triple kinase alpha. J. Biol. Chem. 280(14): 13545-13553
  3. Dong, Z. et al. (2006) The role of histone H3 phosphorylation (Ser10 and Ser28) in cell growth and cell transformation. Mol. Carcinog. 45(6): 416-421

Related products for
Histone H3 pS28 Antibody, anti-human/mouse, REAfinity™

3 products available

Seems like you are coming from USA!
Do you want to visit our website in your country?