FoxP3 is expressed predominantly in regulatory T cells (Tregs) and is a major marker and functional regulator of Treg cell development and function.
1,2
The antibody is suitable for intracellular staining of FoxP3 in human, mouse, and non-human primate cells and subsequent identification and enumeration of these cells by flow cytometry. The FoxP3 Staining Buffer Set is optimized for intracellular staining of FoxP3 with the antibody clone 3G3.

Data and images for FoxP3 Staining Buffer Set

Figures

Figure 1

Human PBMCs were stained with CD4-FITC. Cells were fixed, permeabilized, and intracellularly stained with Anti-FoxP3-PE (A) or Anti-FoxP3-APC (B) using the FoxP3 Staining Buffer Set. Cells were analyzed by flow cytometry.
A:
B:
View details

Figure 1

Human PBMCs were stained with CD4-FITC. Cells were fixed, permeabilized, and intracellularly stained with Anti-FoxP3-PE (A) or Anti-FoxP3-APC (B) using the FoxP3 Staining Buffer Set. Cells were analyzed by flow cytometry.
View details

Figure 1

Human PBMCs were stained with CD4-FITC. Cells were fixed, permeabilized, and intracellularly stained with Anti-FoxP3-PE (A) or Anti-FoxP3-APC (B) using the FoxP3 Staining Buffer Set. Cells were analyzed by flow cytometry.

Figure 2

Mouse splenocytes were stained with CD4-FITC. Cells were fixed, permeabilized, and intracellularly stained with Anti-FoxP3-PE (A) or Anti-FoxP3-APC (B) using the FoxP3 Staining Buffer Set. Cells were analyzed by flow cytometry.
A:
B:
View details

Figure 2

Mouse splenocytes were stained with CD4-FITC. Cells were fixed, permeabilized, and intracellularly stained with Anti-FoxP3-PE (A) or Anti-FoxP3-APC (B) using the FoxP3 Staining Buffer Set. Cells were analyzed by flow cytometry.
View details

Figure 2

Mouse splenocytes were stained with CD4-FITC. Cells were fixed, permeabilized, and intracellularly stained with Anti-FoxP3-PE (A) or Anti-FoxP3-APC (B) using the FoxP3 Staining Buffer Set. Cells were analyzed by flow cytometry.

Specifications for FoxP3 Staining Buffer Set

Overview

FoxP3 is expressed predominantly in regulatory T cells (Tregs) and is a major marker and functional regulator of Treg cell development and function.
1,2
The antibody is suitable for intracellular staining of FoxP3 in human, mouse, and non-human primate cells and subsequent identification and enumeration of these cells by flow cytometry. The FoxP3 Staining Buffer Set is optimized for intracellular staining of FoxP3 with the antibody clone 3G3.

References for FoxP3 Staining Buffer Set

Publications

  1. Scheffold, A: et al. (2018) CD137+CD154- Expression As a Regulatory T Cell (Treg)-Specific Activation Signature for Identification and Sorting of Stable Human Tregs from In Vitro Expansion Cultures. Front Immunol 9
  2. Hori, S. et al. (2003) Control of regulatory T cell development by the transcription factor FoxP3. Science 299: 1057-1061
  3. Walker, M. R. et al. (2003)
    Induction of FoxP3 and acquisition of T regulatory activity by stimulated human CD4
    +
    CD25
    -
    T cells.
    J. Clin. Invest. 112: 1437-1443

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FoxP3 Staining Buffer Set

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