Clone:
REA788
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
ICFC
Alternative names:
AIID, DIETER, IPEX, JM2, PIDX, XPID

Extended validation for FoxP3 Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA788
150D-
3G3++
FJK-16s-
MF-14-
MF23-
Cells were incubated with an excess of purified unconjugated FoxP3 (REA788) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for FoxP3. Thymocytes from C57BL/6 mice were stained with a suitable counterstaining and then fixed and permeabilized using the FoxP3 Staining Buffer Set. Cells were then stained with FoxP3 antibodies. As a control, FoxP3 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for FoxP3. Thymocytes from C57BL/6 mice were stained with a suitable counterstaining and then fixed and permeabilized using the FoxP3 Staining Buffer Set. Cells were then stained with FoxP3 antibodies. As a control, FoxP3 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for FoxP3. Thymocytes from C57BL/6 mice were stained with a suitable counterstaining and then fixed and permeabilized using the FoxP3 Staining Buffer Set. Cells were then stained with FoxP3 antibodies. As a control, FoxP3 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for FoxP3. Thymocytes from C57BL/6 mice were stained with a suitable counterstaining and then fixed and permeabilized using the FoxP3 Staining Buffer Set. Cells were then stained with FoxP3 antibodies. As a control, FoxP3 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for FoxP3. Thymocytes from C57BL/6 mice were stained with a suitable counterstaining and then fixed and permeabilized using the FoxP3 Staining Buffer Set. Cells were then stained with FoxP3 antibodies. As a control, FoxP3 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for FoxP3. Thymocytes from C57BL/6 mice were stained with a suitable counterstaining and then fixed and permeabilized using the FoxP3 Staining Buffer Set. Cells were then stained with FoxP3 antibodies. As a control, FoxP3 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for FoxP3. Thymocytes from C57BL/6 mice were stained with a suitable counterstaining and then fixed and permeabilized using the FoxP3 Staining Buffer Set. Cells were then stained with FoxP3 antibodies. As a control, FoxP3 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for FoxP3. Thymocytes from C57BL/6 mice were stained with a suitable counterstaining and then fixed and permeabilized using the FoxP3 Staining Buffer Set. Cells were then stained with FoxP3 antibodies. As a control, FoxP3 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. The recommended titers of respective antibodies from different suppliers were used.

Specifications for FoxP3 Antibody, anti-mouse, REAfinity™

Overview

Clone REA788 recognizes the forkhead/winged-helix transcriptional regulator FoxP3, also known as forkhead box P3, scurfin, or JM2. FoxP3 is expressed pre-dominantly in regulatory T cells (Tregs) and is a major marker and functional regulator of Treg cell development and function. Mutations in the FoxP3 gene are linked to the autoimmune manifestations observed in the Scurfy mouse and humans with immune dysregulation, polyendocrinopathy, enteropathy, X-linked (IPEX) syndrome. Studies in mice have shown that FoxP3-deficient animals lack Treg cells, whereas overexpression of the FoxP3 protein leads to profound immune suppression.
Additional information: Clone REA788 displays negligible binding to Fc receptors.

Alternative names

AIID, DIETER, IPEX, JM2, PIDX, XPID

Detailed product information

Technical specifications

CloneREA788
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenFoxP3
Alternative names of antigenAIID, DIETER, IPEX, JM2, PIDX, XPID
Molecular mass of antigen [kDa]47
Distribution of antigenT cells
Entrez Gene ID50943
RRIDAB_2651766, AB_2651767, AB_2651768, AB_2651769, AB_2651770, AB_2651771, AB_2651772, AB_2651765

References for FoxP3 Antibody, anti-mouse, REAfinity™

Publications

  1. Sakaguchi, S. et al. (1995) Immunologic self-tolerance maintained by activated T cells expressing IL-2 receptor α-chains (CD25). Breakdown of a single mechanism of self-tolerance causes various autoimmune diseases. J. Immunol. 155: 1151-1164
  2. Hori, S. et al. (2003) Control of regulatory T cell development by the transcription factor FoxP3. Science 299: 1057-1061
  3. Walker, M. R. et al. (2003)
    Induction of FoxP3 and acquisition of T regulatory activity by stimulated human CD4
    +
    CD25
    -
    T cells.
    J. Clin. Invest. 112: 1437-1443
  4. Jaeckel, E. et al. (2005) Antigen-specific FoxP3-transduced T-cells can control established type 1 diabetes. Diabetes 54: 306-310
  5. Ziegler, S. F. (2006) FoxP3: of mice and men. Annu. Rev. Immunol. 24: 209-226
  6. Gavin, M. A. et al. (2006) Single-cell analysis of normal and FOXP3-mutant human T cells: FOXP3 expression without regulatory T cell development. Proc. Natl. Acad. Sci. U.S.A. 103: 6659-6664
  7. Lamprecht, B. et al. (2008) Aberrant expression of the Tʜ2 cytokine IL-21 in Hodgkin lymphoma cells regulates STAT3 signaling and attracts Treg cells via regulation of MIP-3alpha. Blood 112(8): 3339-3347

Related products for
FoxP3 Antibody, anti-mouse, REAfinity™

3 products available