Name: Feeder Removal MicroBeads, mouse
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Product data and images for Feeder Removal MicroBeads, mouse

Figures

Figure 1

Mouse embryonic fibroblasts (mEF) were depleted from a co-culture with mouse embryonic stem (mES) cells using Feeder Removal MicroBeads, an LS Column, and a MidiMACS™ Separator. Flow cytometric analysis with CD15 (SSEA-1)-APC and Anti-Feeder-PE on the MACSQuant® Analyzer indicates that a starting population of 10% mEF was completely removed. The purity of the enriched mES cells was about 99.9%.

Unseparated fraction

After separation

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Figure 1

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Figure 1

Specifications for Feeder Removal MicroBeads, mouse

Overview

Feeder Removal MicroBeads have been developed for the efficient depletion of mouse feeder cells from co-cultures with human or mouse ES and iPS cell lines and can also be used with other, feeder-dependent cell lines. Depletion of feeder cells, e.g. prior to differentiation or gene expression profiling studies, helps to obtain reproducible results.

Detailed product information

Background information

The most prevalent cultivation method for maintenance of mouse and human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells is coculturing on mouse embryonic fibroblast feeder cell layers. Different feeder cell types are employed, among these primary embryonic fibroblasts (mEF) and mEF-derived cell lines (e.g. NIH3T3). Feeder cells are also used in maintenance cultures of primary keratinocytes.

Downstream applications

Depletion of feeder cells improves the reproducibility of many downstream application, including

Gene expression-profiling
Blastocyst injection of ES cells after gene targeting
Differentiation protocols for ES and iPS cells: Removal of feeder cells can improve the responsiveness to developmental cues.

Columns

LS or autoMACS

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Columns.

Resources for Feeder Removal MicroBeads, mouse

Documents and Protocols

Scientific posters

Novel fibroblast-specific marker for rapid, efficient removal of mEFs from stem cell cultures by magnetic separation. ISSCR (2010)

App notes/customer reports

Use of magnetically enriched pluripotent stem cells increases chimerism rate after blastocyst injection and enables the use of inbred ES cell lines for tetraploid complementation. Baskale et al. (2012) MACS&more 14(2).

App notes/customer reports

Data and images for Feeder Removal MicroBeads, mouse

Figures

Figure 1

Unseparated fraction

After separation

View details

Figure 1

View details

Figure 1

Specifications

Specifications for Feeder Removal MicroBeads, mouse

Overview

Detailed product information

Background information

Downstream applications

Depletion of feeder cells improves the reproducibility of many downstream application, including

Gene expression-profiling
Blastocyst injection of ES cells after gene targeting
Differentiation protocols for ES and iPS cells: Removal of feeder cells can improve the responsiveness to developmental cues.

Columns

LS or autoMACS

^®

Columns.

Resources

Resources for Feeder Removal MicroBeads, mouse

Documents and Protocols

Scientific posters

Novel fibroblast-specific marker for rapid, efficient removal of mEFs from stem cell cultures by magnetic separation. ISSCR (2010)

App notes/customer reports

Use of magnetically enriched pluripotent stem cells increases chimerism rate after blastocyst injection and enables the use of inbred ES cell lines for tetraploid complementation. Baskale et al. (2012) MACS&more 14(2).

App notes/customer reports

Increased chimerism after magnetic ES cell enrichment

Protocols

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Feeder Removal MicroBeads, mouse

Feeder Removal MicroBeads, mouse

Product overview

Product data and images for Feeder Removal MicroBeads, mouse

Figures

Figure 1

Figure 1

Figure 1

Specifications for Feeder Removal MicroBeads, mouse

Overview

Detailed product information

Background information

Downstream applications

Columns

Resources for Feeder Removal MicroBeads, mouse

Documents and Protocols

Data and images for Feeder Removal MicroBeads, mouse

Figures

Figure 1

Figure 1

Figure 1

Specifications for Feeder Removal MicroBeads, mouse

Overview

Detailed product information

Background information

Downstream applications

Columns

Resources for Feeder Removal MicroBeads, mouse

Documents and Protocols

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