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A single-cell suspension was prepared using the Epidermis Dissociation Kit, mouse. Langerhans cells were isolated from mouse epidermis single-cell suspension by using the Epidermal Langerhans Cell MicroBead Kit, one MS Column, and a MiniMACS™ Separator. The cells were fluorescently stained with CD207 and CD11c and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
Unseparated fraction | After separation |
Epidermal Langerhans Cell MicroBead Kit, mouseFigure 1A single-cell suspension was prepared using the Epidermis Dissociation Kit, mouse. Langerhans cells were isolated from mouse epidermis single-cell suspension by using the Epidermal Langerhans Cell MicroBead Kit, one MS Column, and a MiniMACS™ Separator. The cells were fluorescently stained with CD207 and CD11c and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. | Epidermal Langerhans Cell MicroBead Kit, mouseFigure 1A single-cell suspension was prepared using the Epidermis Dissociation Kit, mouse. Langerhans cells were isolated from mouse epidermis single-cell suspension by using the Epidermal Langerhans Cell MicroBead Kit, one MS Column, and a MiniMACS™ Separator. The cells were fluorescently stained with CD207 and CD11c and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
A single-cell suspension was prepared using the Epidermis Dissociation Kit, mouse. Langerhans cells were isolated from mouse epidermis single-cell suspension by using the Epidermal Langerhans Cell MicroBead Kit, one MS Column, and a MiniMACS™ Separator. The cells were fluorescently stained with CD207 and CD11c and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
Unseparated fraction | After separation |
Epidermal Langerhans Cell MicroBead Kit, mouseFigure 1A single-cell suspension was prepared using the Epidermis Dissociation Kit, mouse. Langerhans cells were isolated from mouse epidermis single-cell suspension by using the Epidermal Langerhans Cell MicroBead Kit, one MS Column, and a MiniMACS™ Separator. The cells were fluorescently stained with CD207 and CD11c and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. | Epidermal Langerhans Cell MicroBead Kit, mouseFigure 1A single-cell suspension was prepared using the Epidermis Dissociation Kit, mouse. Langerhans cells were isolated from mouse epidermis single-cell suspension by using the Epidermal Langerhans Cell MicroBead Kit, one MS Column, and a MiniMACS™ Separator. The cells were fluorescently stained with CD207 and CD11c and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
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