Epidermal Langerhans Cell MicroBead Kit, mouse

Name: Epidermal Langerhans Cell MicroBead Kit, mouse
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Epidermal Langerhans Cell MicroBead Kit, mouse

The Epidermal Langerhans Cell MicroBead Kit is designed for the positive selection or depletion of Langerhans cells (LCs) from single-cell suspensions of the epidermis.

Product data and images for Epidermal Langerhans Cell MicroBead Kit, mouse

Figures

Figure 1

A single-cell suspension was prepared using the Epidermis Dissociation Kit, mouse. Langerhans cells were isolated from mouse epidermis single-cell suspension by using the Epidermal Langerhans Cell MicroBead Kit, one MS Column, and a MiniMACS™ Separator. The cells were fluorescently stained with CD207 and CD11c and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Unseparated fraction

After separation

Figure 1

A single-cell suspension was prepared using the Epidermis Dissociation Kit, mouse. Langerhans cells were isolated from mouse epidermis single-cell suspension by using the Epidermal Langerhans Cell MicroBead Kit, one MS Column, and a MiniMACS™ Separator. The cells were fluorescently stained with CD207 and CD11c and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Figure 1

A single-cell suspension was prepared using the Epidermis Dissociation Kit, mouse. Langerhans cells were isolated from mouse epidermis single-cell suspension by using the Epidermal Langerhans Cell MicroBead Kit, one MS Column, and a MiniMACS™ Separator. The cells were fluorescently stained with CD207 and CD11c and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for Epidermal Langerhans Cell MicroBead Kit, mouse

Overview

The Epidermal Langerhans Cell MicroBead Kit is designed for the positive selection or depletion of Langerhans cells (LCs) from single-cell suspensions of the epidermis.

Detailed product information

Background information

Epidermal LCs are the principal dendritic cell population in the epidermis and play a pivotal role in the initiation and maintenance of primary immune responses in the skin. Immature LCs are highly specialized in antigen uptake.

In vivo

LCs exposed to pathogens migrate from the skin to the draining lymph nodes, where they elicit antigen-specific T cell responses.

^1-5

Applications

Positive selection or depletion of mouse epidermal Langerhans cells from single-cell suspension prepared from epidermis using the Epidermis Dissociation Kit, mouse.

Columns

For positive selection: MS, LS, or autoMACS

^®

Columns. For depletion: LD or autoMACS Columns.

References for Epidermal Langerhans Cell MicroBead Kit, mouse

Publications

Takahara, K. et al. (2002) Identification and expression of mouse Langerin (CD207) in dendritic cells. Int. Immunol. 14: 433-444
Romani, N. et al. (2003) Langerhans cells - dendritic cells of the epidermis. APMIS 111: 725-740
Schuler, G. and Steinman, R. M. (1985)
Murine epidermal Langerhans cells mature into potent immunostimulatory dendritic cells
in vitro
.
J. Exp. Med. 161: 526-546
Merad, M. et al. (2008) Origin, homeostasis and function of Langerhans cells and other langerin-expressing dendritic cells. Nat. Rev. Immunol. 8: 935-947
Kaplan, D. H. et al. (2008) Insights into Langerhans cell function from Langerhans cell ablation models. Eur. J. Immunol. 38: 2369-2376

Data and images

Data and images for Epidermal Langerhans Cell MicroBead Kit, mouse

Figures

Figure 1

A single-cell suspension was prepared using the Epidermis Dissociation Kit, mouse. Langerhans cells were isolated from mouse epidermis single-cell suspension by using the Epidermal Langerhans Cell MicroBead Kit, one MS Column, and a MiniMACS™ Separator. The cells were fluorescently stained with CD207 and CD11c and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Unseparated fraction

After separation

Figure 1

A single-cell suspension was prepared using the Epidermis Dissociation Kit, mouse. Langerhans cells were isolated from mouse epidermis single-cell suspension by using the Epidermal Langerhans Cell MicroBead Kit, one MS Column, and a MiniMACS™ Separator. The cells were fluorescently stained with CD207 and CD11c and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Figure 1

A single-cell suspension was prepared using the Epidermis Dissociation Kit, mouse. Langerhans cells were isolated from mouse epidermis single-cell suspension by using the Epidermal Langerhans Cell MicroBead Kit, one MS Column, and a MiniMACS™ Separator. The cells were fluorescently stained with CD207 and CD11c and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications

Specifications for Epidermal Langerhans Cell MicroBead Kit, mouse

Overview

The Epidermal Langerhans Cell MicroBead Kit is designed for the positive selection or depletion of Langerhans cells (LCs) from single-cell suspensions of the epidermis.

Detailed product information

Background information

Epidermal LCs are the principal dendritic cell population in the epidermis and play a pivotal role in the initiation and maintenance of primary immune responses in the skin. Immature LCs are highly specialized in antigen uptake.

In vivo

LCs exposed to pathogens migrate from the skin to the draining lymph nodes, where they elicit antigen-specific T cell responses.

^1-5

Applications

Positive selection or depletion of mouse epidermal Langerhans cells from single-cell suspension prepared from epidermis using the Epidermis Dissociation Kit, mouse.

Columns

For positive selection: MS, LS, or autoMACS

^®

Columns. For depletion: LD or autoMACS Columns.

References

References for Epidermal Langerhans Cell MicroBead Kit, mouse

Publications

Takahara, K. et al. (2002) Identification and expression of mouse Langerin (CD207) in dendritic cells. Int. Immunol. 14: 433-444
Romani, N. et al. (2003) Langerhans cells - dendritic cells of the epidermis. APMIS 111: 725-740
Schuler, G. and Steinman, R. M. (1985)
Murine epidermal Langerhans cells mature into potent immunostimulatory dendritic cells
in vitro
.
J. Exp. Med. 161: 526-546
Merad, M. et al. (2008) Origin, homeostasis and function of Langerhans cells and other langerin-expressing dendritic cells. Nat. Rev. Immunol. 8: 935-947
Kaplan, D. H. et al. (2008) Insights into Langerhans cell function from Langerhans cell ablation models. Eur. J. Immunol. 38: 2369-2376

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Epidermal Langerhans Cell MicroBead Kit, mouse

Epidermal Langerhans Cell MicroBead Kit, mouse

Product data and images for Epidermal Langerhans Cell MicroBead Kit, mouse

Figures

Figure 1

Figure 1

Figure 1

Specifications for Epidermal Langerhans Cell MicroBead Kit, mouse

Overview

Detailed product information

Background information

Applications

Columns

References for Epidermal Langerhans Cell MicroBead Kit, mouse

Publications

Data and images

Data and images for Epidermal Langerhans Cell MicroBead Kit, mouse

Figures

Figure 1

Figure 1

Figure 1

Specifications

Specifications for Epidermal Langerhans Cell MicroBead Kit, mouse

Overview

Detailed product information

Background information

Applications

Columns

References

References for Epidermal Langerhans Cell MicroBead Kit, mouse

Publications

Related products for
Epidermal Langerhans Cell MicroBead Kit, mouse

5 products available | view all view less

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Epidermal Langerhans Cell MicroBead Kit, mouse

Epidermal Langerhans Cell MicroBead Kit, mouse

Product data and images for Epidermal Langerhans Cell MicroBead Kit, mouse

Figures

Figure 1