The Epidermal Langerhans Cell MicroBead Kit is designed for the positive selection or depletion of Langerhans cells (LCs) from single-cell suspensions of the epidermis.

Data and images for Epidermal Langerhans Cell MicroBead Kit, mouse

Figures

Figure 1

A single-cell suspension was prepared using the Epidermis Dissociation Kit, mouse. Langerhans cells were isolated from mouse epidermis single-cell suspension by using the Epidermal Langerhans Cell MicroBead Kit, one MS Column, and a MiniMACS™ Separator. The cells were fluorescently stained with CD207 and CD11c and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Unseparated fraction
After separation
View details

Figure 1

A single-cell suspension was prepared using the Epidermis Dissociation Kit, mouse. Langerhans cells were isolated from mouse epidermis single-cell suspension by using the Epidermal Langerhans Cell MicroBead Kit, one MS Column, and a MiniMACS™ Separator. The cells were fluorescently stained with CD207 and CD11c and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

A single-cell suspension was prepared using the Epidermis Dissociation Kit, mouse. Langerhans cells were isolated from mouse epidermis single-cell suspension by using the Epidermal Langerhans Cell MicroBead Kit, one MS Column, and a MiniMACS™ Separator. The cells were fluorescently stained with CD207 and CD11c and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for Epidermal Langerhans Cell MicroBead Kit, mouse

Overview

The Epidermal Langerhans Cell MicroBead Kit is designed for the positive selection or depletion of Langerhans cells (LCs) from single-cell suspensions of the epidermis.

Detailed product information

Background information

Epidermal LCs are the principal dendritic cell population in the epidermis and play a pivotal role in the initiation and maintenance of primary immune responses in the skin. Immature LCs are highly specialized in antigen uptake.
In vivo
LCs exposed to pathogens migrate from the skin to the draining lymph nodes, where they elicit antigen-specific T cell responses.
1-5

Applications

Positive selection or depletion of mouse epidermal Langerhans cells from single-cell suspension prepared from epidermis using the Epidermis Dissociation Kit, mouse.

Columns

For positive selection: MS, LS, or autoMACS
®
Columns. For depletion: LD or autoMACS Columns.

References for Epidermal Langerhans Cell MicroBead Kit, mouse

Publications

  1. Takahara, K. et al. (2002) Identification and expression of mouse Langerin (CD207) in dendritic cells. Int. Immunol. 14: 433-444
  2. Romani, N. et al. (2003) Langerhans cells - dendritic cells of the epidermis. APMIS 111: 725-740
  3. Schuler, G. and Steinman, R. M. (1985)
    Murine epidermal Langerhans cells mature into potent immunostimulatory dendritic cells
    in vitro
    .
    J. Exp. Med. 161: 526-546
  4. Merad, M. et al. (2008) Origin, homeostasis and function of Langerhans cells and other langerin-expressing dendritic cells. Nat. Rev. Immunol. 8: 935-947
  5. Kaplan, D.H. et al. (2008) Insights into Langerhans cell function from Langerhans cell ablation models. Eur. J. Immunol. 38: 2369-2376

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