The Embryoid Body Dissociation Kit was developed for standardized and reproducible dissociation of
in vitro
generated EBs or pluripotent stem cell-derived neurospheres. This convenient and time saving kit was optimized for high yields, high cell viability and high reproducibility.

Specifications for Embryoid Body Dissociation Kit, human and mouse

Overview

The Embryoid Body Dissociation Kit was developed for standardized and reproducible dissociation of
in vitro
generated EBs or pluripotent stem cell-derived neurospheres. This convenient and time saving kit was optimized for high yields, high cell viability and high reproducibility.

Detailed product information

Background information

Embryoid Body (EB) formation is a crucial step in many ES or iPS cell differentiation protocols. Viable single-cell suspensions of EBs are a prerequisite for subsequent cell analysis or isolation of specific cell populations within the EB.

Downstream applications

  • Isolation of EB-derived cell populations using e.g. MACS Cell Separation Technology
  • Cultivation of EB-derived cells
  • Phenotyping or enumeration of individual EB-derived cell populations by flow cytometry

Resources for Embryoid Body Dissociation Kit, human and mouse

Documents and Protocols

References for Embryoid Body Dissociation Kit, human and mouse

Publications

  1. Niwa, A. et al. (2011) A novel serum-free monolayer culture for orderly hematopoietic differentiation of human pluripotent cells via mesodermal progenitors. PLoS One 6: e22261

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