The Diamond CD34 Isolation Kit has been specifically developed for the isolation of highly pure Lin
CD34
+
hematopoietic stem cells from peripheral blood
1
, bone marrow
2,3
or cord blood.

Data and images for Diamond CD34 Isolation Kit, human

Figures

Figure 1

CD34
+
Lin
cells were isolated from human PBMCs using the Diamond CD34 Isolation Kit, an LD Column, two MS Columns, a MidiMACS™ Separator, and a MiniMACS™ Separator. Cells were fluorescently stained with CD34-FITC, CD45-APC, and Anti-Biotin-PE and analyzed using the MACSQuant® Analyzer.
PBMCs before separation
Lineage-negative fraction
View details

Figure 1

CD34
+
Lin
cells were isolated from human PBMCs using the Diamond CD34 Isolation Kit, an LD Column, two MS Columns, a MidiMACS™ Separator, and a MiniMACS™ Separator. Cells were fluorescently stained with CD34-FITC, CD45-APC, and Anti-Biotin-PE and analyzed using the MACSQuant® Analyzer.
View details

Figure 1

CD34
+
Lin
cells were isolated from human PBMCs using the Diamond CD34 Isolation Kit, an LD Column, two MS Columns, a MidiMACS™ Separator, and a MiniMACS™ Separator. Cells were fluorescently stained with CD34-FITC, CD45-APC, and Anti-Biotin-PE and analyzed using the MACSQuant® Analyzer.
Isolated CD34
+
Lin
cells
View details

Figure 1

CD34
+
Lin
cells were isolated from human PBMCs using the Diamond CD34 Isolation Kit, an LD Column, two MS Columns, a MidiMACS™ Separator, and a MiniMACS™ Separator. Cells were fluorescently stained with CD34-FITC, CD45-APC, and Anti-Biotin-PE and analyzed using the MACSQuant® Analyzer.

Specifications for Diamond CD34 Isolation Kit, human

Overview

The Diamond CD34 Isolation Kit has been specifically developed for the isolation of highly pure Lin
CD34
+
hematopoietic stem cells from peripheral blood
1
, bone marrow
2,3
or cord blood.

Detailed product information

Background information

The CD34 antigen is the most common marker for the isolation of hematopoietic stem and progenitor cells, but is also expressed on some lineage-committed cells. With the Diamond CD34 Isolation Kit, lineage-positive cells are removed in order to obtain pure, primitive CD34
+
HSCs.

Detailed separation procedure

The Diamond CD34 MicroBead Kit includes a lineage depletion followed by a CD34 isolation. In the first step, cells are labeled with a cocktail of biotin-conjugated antibodies against lineage-specific antigens. These cells are subsequently labeled with Anti-Biotin MicroBeads. Lineage-negative stem and progenitor cells are obtained by depletion of the magnetically labeled cells. Then CD34
+
cells are isolated from the lineage-negative stem and progenitor cells using CD34 MicroBeads. The resulting population obtained are CD34
+
Lin
stem cells.

Downstream applications

Purified CD34
+
Lin
-
stem cells can be used for many applications, including:
  • gene or miRNA expression profiling
  • characterization of undifferentiated stem cells and their developmental potential
  • stimulation of proliferation and maturation by cytokines
  • HSC-CFU assays
  • adoptive transfer and hematopoietic reconstitution

Columns

For positive selection: MS or autoMACS
®
Columns. For depletion: LD or autoMACS Columns.

References for Diamond CD34 Isolation Kit, human

Publications

  1. Wu, W.-C. et al. (2014) Circulating hematopoietic stem and progenitor cells are myeloid-biased in cancer patients. Proc. Natl. Acad. Sci. U.S.A. 111: 4221-4226
  2. Zhang, S. et al. (2012) Matrine induces apoptosis in human acute myeloid leukemia cells via the mitochondrial pathway and Akt inactivation. PLoS One 7(10): e46853
  3. Nair, R.R. et al. (2012) Potentiation of Nilotinib-mediated cell death in the context of the bone marrow microenvironment requires a promiscuous JAK inhibitor in CML. Leuk. Res. 36(6): 756-763

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