Clone:
REA1101
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
SELPLG, CD162, PSGL-1, PSGL1

Extended validation for CLA Antibody, anti-human, REAfinity™

Sensitivity

Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CLA (REA1101). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CLA (REA1101). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CLA (REA1101). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CLA Antibody, anti-human, REAfinity™

Overview

Clone REA1101 recognizes the cutaneous lymphocyte-associated antigen (CLA), a specialized glycoform of P-selectin glycoprotein ligand-1 (PSGL-1). It serves as a ligand for selectins, including CD62E (ELAM-1) and CD62L (LECAM-1). CLA is a unique skin-homing receptor and is predominantly found on a minor subset of human T cells that infiltrate the skin. This post-translational modification of PSGL-1 is thought to serve as a mechanism to regulate tissue-specific homing of CD4
+
and CD8
+
memory/effector T cells from peripheral blood to the skin, which plays an essential role during many inflammatory and certain malignant skin diseases. In peripheral blood, CLA is not only found on skin-homing memory/effector T cells, but is also found to be expressed on memory/effector B cells, NK cells, blood dendritic cells, and on monocytes. CLA is furthermore found on Langerhans cells in the skin.
Additional information: Clone REA1101 displays negligible binding to Fc receptors.

Alternative names

SELPLG, CD162, PSGL-1, PSGL1

Detailed product information

Technical specifications

CloneREA1101
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCLA
Alternative names of antigenSELPLG, CD162, PSGL-1, PSGL1
Molecular mass of antigen [kDa]39
Distribution of antigendendritic cells, Langerhans cells, monocytes, NK cells, T cells, epithelial cells, memory B cells, effector T cells, memory T cells
Entrez Gene ID6404
RRIDAB_2733851, AB_2733409, AB_2733410, AB_2733047, AB_2733048, AB_2733850

References for CLA Antibody, anti-human, REAfinity™

Publications

  1. Santamaria Babi, L. F. et al. (1995) Migration of skin-homing T cells across cytokine-activated human endothelial cell layers involves interaction of the cutaneous lymphocyte-associated antigen (CLA), the very late antigen-4 (VLA-4), and the lymphocyte function-associated antigen-1 (LFA-1). J. Immunol. 154: 1543-1550
  2. Fuhlbrigge, R. C. et al. (1997) Cutaneous lymphocyte antigen is a specialized form of PSGL-1 expressed on skin-homing T cells. Nature 389: 978-981
  3. Yoshino, T. et al. (1999) Cutaneous lymphocyte antigen is expressed on memory/effector B cells in the peripheral blood and monocytoid B cells in the lymphoid tissues. Cell. Immunol. 197: 39-45
  4. Takahashi, R. et al. (2003)
    In vitro
    differentiation from naive to mature E-selectin binding CD4 T cells: acquisition of skin-homing properties occurs independently of cutaneous lymphocyte antigen expression.
    J. Immunol. 171: 5769-5777
  5. Bos, J. D. et al. (1993) Skin-homing T lymphocytes: detection of cutaneous lymphocyte-associated antigen (CLA) by HECA.452 in normal human skin. Arch. Dermatol. Res. 285(179): 179-183

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