MACS GMP Recombinant Human TNF-α

MACS GMP Recombinant Human TNF-α

Tumor necrosis factor alpha (TNF-α) has a broad spectrum of biological activities. In addition to its central role in inflammation TNF-α is involved in regulating apoptotic cell death, cellular proliferation, and differentiation.
MACS GMP Recombinant Human TNF-α is designed for
ex vivo
cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.

Disclaimer

MACS GMP Products are for research use and
ex vivo
cell culture processing only, and are not intended for human
in vivo
applications. For regulatory status in the USA, please contact your local representative.

Background information

TNF-α is mainly produced by activated monocytes and macrophages, but also by a broad variety of other cell types including lymphoid cells, mast cells, endothelial cells, fibroblasts, and astrocytes. Beside its primary role in immune regulation, TNF-α promotes angiogenesis, bone resorption, and thrombotic processes.

Applications

MACS GMP Recombinant Human TNF-α can be used for a variety of applications, including the
ex vivo
generation of human dendritic cells from enriched CD14
+
monocyte populations.
2–4

Quality statement

MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.

Biological activity

  • The specific activity is determined by cytotoxicity assay using L929 cells provided by the German Collection of Microorganisms and Cell Cultures (DSMZ) in the presence of 1 μg/mL actinomycin D according to Baarsch
    et al
    .
    1
    The cytotoxicity assay was calibrated with the international standard for human TNF-α (NIBSC code 88/786) provided by the National Institute for Biological Standards and Control.
  • Specific activity: ≥ 2×
    10
    7
    IU/mg
  • Certificates of Analysis (CoA)

    Please follow this
    link
    to search for certificates by lot number.
Product options: 1

Product information

Size
Order no.
Price

MACS GMP Recombinant Human TNF-α

  • E. coli
  • For research use and
    ex vivo
    cell processing only
25 µg
170-076-103
EUR 367,00