Clone:
REA921
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
CCR8, CCR-8, CC-CKR-8

Extended validation for CDw198 (CCR8) Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA921
1055C++
SA214G2++
Cells were incubated with an excess of purified unconjugated CDw198 (CCR8) (REA921) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CDw198. Thymocytes from C57BL/6 mice were stained with CDw198 antibodies and with a suitable counterstaining. As a control, CDw198 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CDw198. Thymocytes from C57BL/6 mice were stained with CDw198 antibodies and with a suitable counterstaining. As a control, CDw198 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CDw198. Thymocytes from C57BL/6 mice were stained with CDw198 antibodies and with a suitable counterstaining. As a control, CDw198 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CDw198. Thymocytes from C57BL/6 mice were stained with CDw198 antibodies and with a suitable counterstaining. As a control, CDw198 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CDw198 (CCR8) (REA921). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CDw198 (CCR8) (REA921). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CDw198 (CCR8) (REA921). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CDw198 (CCR8) Antibody, anti-mouse, REAfinity™

Overview

Clone REA921 recognizes the mouse CDw198 antigen, also known as C-C chemokine receptor type 8 (CCR8). CDw198, a G-protein coupled receptor with seven transmembrane regions, is a member of the beta chemokine receptor family, which binds to the T cell activation gene 3 (TCA-3) and CCL1. CDw198 is primarily found in thymus. It is expressed in activated Tʜ2-polarized cells and NK1.1
+
CD4
+
T cells, but also in subsets of monocytes, macrophages, and monocyte-derived dendritic cells. CDw198 plays a role in migration of various cell types into inflammatory sites.
Additional information: Clone REA921 displays negligible binding to Fc receptors.

Alternative names

CCR8, CCR-8, CC-CKR-8

Detailed product information

Technical specifications

CloneREA921
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenCDw198 (CCR8)
Alternative names of antigenCCR8, CCR-8, CC-CKR-8
Molecular mass of antigen [kDa]40
Distribution of antigendendritic cells, macrophages, monocytes, NK cells, T cells, CD4+ T cells, Th2 cells
Entrez Gene ID12776
RRIDAB_2751259, AB_2751260, AB_2751921, AB_2751922, AB_2751261

References for CDw198 (CCR8) Antibody, anti-mouse, REAfinity™

Publications

  1. Goya, I. et al. (1998) Identification of CCR8 as the specific receptor for the human beta-chemokine I-309: cloning and molecular characterization of murine CCR8 as the receptor for TCA-3. J. Immunol. 160(4): 1975-1981
  2. Zingoni, A. et al. (1998) The chemokine receptor CCR8 is preferentially expressed in Tʜ2 but not Tʜ1 cells. J. Immunol. 161(2): 547-551
  3. Islam, S. A. et al. (2011)
    Mouse CCL8, a CCR8 agonist, promotes atopic dermatitis by recruiting IL-5
    +
    Tʜ2 cells.
    Nat. Immunol. 12(2): 167-177

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