Clone:
REA1174
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
HBA71, MIC2, MIC2X, MIC2Y, MSK5X, E2

Extended validation for CD99 Antibody, anti-human, REAfinity™

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of CD99 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD99-PE (REA1174, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Fluorescence microscopy image of CD99 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD99-PE (REA1174, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of CD99 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD99-PE (REA1174, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of CD99 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD99-PE, clone (REA1174). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of CD99 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD99-PE, clone (REA1174). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD99 Antibody, anti-human, REAfinity™

Overview

Clone REA1174 recognizes the human CD99 antigen, a 32 kDa type I single-chain transmembrane sialoglycoprotein which is also known as E2 antigen or MIC2. The expression level of CD99 is particularly high in thymocytes, T cells, T cell leukemias and lymphomas, subsets of mature plasma cells in the medullary cord of reactive lymph nodes, pancreatic islet cells, granulosa cells of the ovary, Sertoli cells of the testes, but absent on granulocytes. It is involved in T cell–adhesion processes and in spontaneous rosette formation with erythrocytes. CD99 plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane and it acts at the same site as, but independently of, PECAM1.
Clone REA1174 displays negligible binding to Fc receptors.

Alternative names

HBA71, MIC2, MIC2X, MIC2Y, MSK5X, E2

Detailed product information

Technical specifications

CloneREA1174
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD99
Alternative names of antigenHBA71, MIC2, MIC2X, MIC2Y, MSK5X, E2
Molecular mass of antigen [kDa]19
Distribution of antigenT cells, thymocytes, plasma cells, leukemia cells, pan T cells, other
Entrez Gene ID4267
RRIDAB_2784329, AB_2784328, AB_2784327, AB_2784332, AB_2784331, AB_2784330

References for CD99 Antibody, anti-human, REAfinity™

Publications

  1. Schenkel, A. R. et al. (2002) CD99 plays a major role in the migration of monocytes through endothelial junctions. Nat. Immunol. 3(2): 143-150
  2. Gelin, C. et al. (1989) The E2 antigen, a 32 kd glycoprotein involved in T-cell adhesion processes, is the MIC2 gene product. EMBO J. 8(11): 3253-3259
  3. Edlund, K. et al. (2012) CD99 is a novel prognostic stromal marker in non-small cell lung cancer. Int. J. Cancer 131(10): 2264-2273

Related products for
CD99 Antibody, anti-human, REAfinity™

3 products available

Seems like you are coming from USA!
Do you want to visit our website in your country?