Clone:
REA234
Type of antibody:
Functional-grade antibodies, Recombinant antibodies, Primary antibodies
Isotype:
recombinant human IgG1
Applications:
FA, FC, MICS, IF, IHC
Alternative names:
FCAR, CTB-61M7.2, FcαRI

Extended validation for CD89 Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA234
A59-
MIP8a+
Cells were incubated with an excess of purified unconjugated CD89 (REA234) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.

Specifications for CD89 Antibody, anti-human, REAfinity™

Overview

Clone REA234 recognizes CD89, the human myeloid IgA Fc receptor. CD89 is a glycosylated transmembrane protein that binds both IgA subclasses (IgA1 and IgA2) and is closely related to the KIR and MIR proteins rather than to other human Fc receptors. CD89 itself lacks the canonical signal transduction domains, however it associates with the FcR γ-chain with an immunoreceptor tyrosine-based activation (ITAM) motif, which allows transduction of activation signal upon ligand binding. FcαRI alone mediates endocytosis and recyling of IgA. Expression of CD89 is found on neutrophils, eosinophils, monocytes/macrophages, dendritic cells, and Kupffer cells.
Additional information: Clone REA234 displays negligible binding to Fc receptors.

Alternative names

FCAR, CTB-61M7.2, FcαRI

Detailed product information

Technical specifications

CloneREA234
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies, Functional-grade antibodies
Specieshuman
AntigenCD89
Alternative names of antigenFCAR, CTB-61M7.2, FcαRI
Distribution of antigendendritic cells, macrophages, monocytes, eosinophils
Entrez Gene ID2204
RRIDAB_2659459, AB_2659460, AB_2659463, AB_2659464, AB_2659465, AB_2659466, AB_2659467, AB_2659468, AB_2659469, AB_2659470, AB_2921843, AB_2921824, AB_2905140, AB_2905139, AB_2921963, AB_2921943, AB_2659458

Resources for CD89 Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD89 Antibody, anti-human, REAfinity™

Publications

  1. Wu, J. et al. (2007) FcαRI (CD89) alleles determine the proinflammatory potential of serum IgA. J Immunol 178: 3973-3982
  2. Morton, H. C. et al. (1995) Functional association between the human myeloid immunoglobulin A Fc receptor (CD89) and FcR chain. J. Biol. Chem. 270: 29781-29787

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