Clone:
FM95
Type of antibody:
Primary antibodies
Isotype:
mouse IgG1κ, mouse IgG1
Applications:
FC, MC
Alternative names:
B7-2, B70, CD28LG2, LAB72

Extended validation for CD86 Antibody, anti-human

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD86. Human peripheral blood mononuclear cells (PBMCs) were stained with CD86 antibodies and with a suitable counterstaining. As a control, CD86 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD86. Human peripheral blood mononuclear cells (PBMCs) were stained with CD86 antibodies and with a suitable counterstaining. As a control, CD86 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD86. Human peripheral blood mononuclear cells (PBMCs) were stained with CD86 antibodies and with a suitable counterstaining. As a control, CD86 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD86. Human peripheral blood mononuclear cells (PBMCs) were stained with CD86 antibodies and with a suitable counterstaining. As a control, CD86 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD86. Human peripheral blood mononuclear cells (PBMCs) were stained with CD86 antibodies and with a suitable counterstaining. As a control, CD86 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD86. Human peripheral blood mononuclear cells (PBMCs) were stained with CD86 antibodies and with a suitable counterstaining. As a control, CD86 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD86. Human peripheral blood mononuclear cells (PBMCs) were stained with CD86 antibodies and with a suitable counterstaining. As a control, CD86 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD86 (FM95). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD86 (FM95). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD86 (FM95). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD86 Antibody, anti-human

Overview

CD86, also known as B7-2 or B70, is an 80 kDa molecule and a member of the immunoglobulin superfamily. Together with CD80 (B7-1) it belongs to the B7 family of costimulatory molecules. CD86 is expressed on activated B and T cells, dendritic cells (DCs), and monocytes/macrophages. The interaction of CD86 with its ligands CD28 and CD152 (CTLA- 4) plays a critical role in induction and regulation of immune responses, e.g., cross-talk between T and B cells, T cell costimulation, or immunoglobulin class-switching. Binding of CD86 to CD28 on T cells results in transduction of costimulatory signals for activation or proliferation of T cells, or cytokine production.

Alternative names

B7-2, B70, CD28LG2, LAB72

Detailed product information

Technical specifications

CloneFM95
Clonalitymonoclonal
Isotypemouse IgG1κ, mouse IgG1
Isotype controlIsotype Control Antibody, mouse IgG1
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman, non-human primate
Cross-reactivity
rhesus monkey (
Macaca mulatta
)
,
cynomolgus monkey (
Macaca fascicularis
)
AntigenCD86
Alternative names of antigenB7-2, B70, CD28LG2, LAB72
Molecular mass of antigen [kDa]35
Distribution of antigenB cells, dendritic cells, endothelial cells, Langerhans cells, monocytes, T cells
Entrez Gene ID942
RRIDAB_2726452, AB_2726175, AB_2659849, AB_2751132, AB_2733842, AB_2733843, AB_2726451, AB_2726174, AB_2733952, AB_2733953, AB_2751199, AB_2751133, AB_2801681, AB_2801678, AB_2751198

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