Clone:
REA221
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
4F9, C33, GR15, IA4, KAI1, R2, SAR2, ST6, TSPAN27

Extended validation for CD82 Antibody, anti-human, REAfinity™

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of CD82 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD82-PE (REA221, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Fluorescence microscopy image of CD82 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD82-PE (REA221, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of CD82 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD82-PE (REA221, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of CD82 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD82-PE, clone (REA221). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of CD82 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD82-PE, clone (REA221). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD82 Antibody, anti-human, REAfinity™

Overview

Clone REA221 recognizes CD82, a member of the four transmembrane domain, tetraspanin family. Tetraspanins contain short, cytoplasmic amino and carboxyl termini and two extracellular loops of unequal sizes and can be distinguished from other membrane proteins, with same topology, due to conserved residues within the transmembrane domains and in the larger extracellular loops. CD82 interacts with other tretaspanins and membrane proteins, thus regulating several functions such as signal transduction. On the surface of tumor cells, CD82 interacts with integrins and is suggested to serve as a supressor of tumor metastasis. In addition, on immune cells such as T cells, CD82 associates with CD81, CD4, or CD8 and class II major histocompatibility complex (MHC), components of the antigen-processing and presentation pathway and tetraspanin CD63, in dendritic cells. Expression of CD82 is found on dendritic cells, monocytes, granulocytes, lymphocytes, epithelial, and endothelial cells.
Additional information: Clone REA221 displays negligible binding to Fc receptors.

Alternative names

4F9, C33, GR15, IA4, KAI1, R2, SAR2, ST6, TSPAN27

Detailed product information

Technical specifications

CloneREA221
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD82
Alternative names of antigen4F9, C33, GR15, IA4, KAI1, R2, SAR2, ST6, TSPAN27
Distribution of antigendendritic cells, granulocytes, monocytes, lymphocytes
RRIDAB_2876981, AB_2659298, AB_2659299, AB_2659300, AB_2659301, AB_2659302, AB_2659303, AB_2876980

References for CD82 Antibody, anti-human, REAfinity™

Publications

  1. Lagaudriere-Gesbert, C. et al. (1997) The tetraspanin protein CD82 associates with both free HLA class I heavy chain and heterodimeric beta 2-microglobulin complexes. J. Immunol. 158: 2790 -2797
  2. Zhang, X. A. et al. (2003) EWI2/PGRL associates with the metastasis suppressor KAI1/CD82 and inhibits the migration of prostate cancer cells. Cancer Res. 63: 2665-2674

Related products for
CD82 Antibody, anti-human, REAfinity™

3 products available

Seems like you are coming from USA!
Do you want to visit our website in your country?