Clone:
BW135/80
Type of antibody:
Primary antibodies
Isotype:
mouse IgG2a
Alternative names:
Leu-2, Ly-2, T8

Specifications for CD8 antibodies

Overview

BW135/80 recognizes the human CD8 antigen which is strongly expressed on human cytotoxic T cells and thymocytes, and is also expressed on a subset of NK cells. The CD8 antigen is a disulfide-linked dimer that exists either as a CD8α homodimer or as a CD8α/β heterodimer. CD8 acts as a coreceptor for the T cell receptor and binds to the MHC Class I molecule. The CD8 antibody recognizes the α-subunit of the antigen.

Applications

Reagent can be used for immunophenotyping by flow cytometry. Abnormal numbers of cells expressing this antigen or aberrant expression levels of the antigen can be expected in some disease states. It is important to understand the normal expression pattern for this antigen and its relationship to expression of other relevant antigens in order to perform appropriate analysis.
Expression of CD8 may be used as aid to diagnostic in the characterization of samples from individuals suspected with hematologic neoplasia.

Alternative names

Leu-2, Ly-2, T8

Detailed product information

Technical specifications

CloneBW135/80
Isotypemouse IgG2a
Type of antibodyPrimary antibodies
Specieshuman
AntigenCD8
Alternative names of antigenLeu-2, Ly-2, T8
Molecular mass of antigen [kDa]45
Distribution of antigendendritic cells, NK cells, T cells

Resources for CD8 antibodies

Certificates

Please follow this
link
to download the Certificate of Conformity (CoC) by lot number.

References for CD8 antibodies

Publications

  1. Stelzer GT et al. (1997) U.S.-Canadian consensus recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry: standardization and validation of laboratory procedures. Cytometry 30(5): 214-230
  2. Rothe, G et al. (2012) Consensus protocol for the flow cytometric Immunophenotyping of hematopoietic malignancies. Leukemia 10(5): 877-895
  3. van Dongen, J. J. M. et al. (2012) EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia 26(9): 1908-1975
  4. Jonker, M. et al. (1989) Reactivity of mAb specific for human CD markers with rhesus monkey leucocytes. Oxford, Oxford University Press (Leukocyte Typing IV): 1058-1063
  5. Clinical and Laboratory Standards Institute (CLSI) (2007) Clinical Flow Cytometric Analysis of Neoplastic Hematolympoid Cells, CLSI document H43-A2 (ISBN 1-56238-635-2) CLSI; Approved Guideline - Second Edition
  6. Clinical and Laboratory Standards Institute (CLSI) (2007) Enumeration of immunologically defined cell populations by flow cytometry, CLSI document H42-A2 (ISBN 1-56238-640-9) CLSI; Approved Guideline - Second Edition
  7. Wood BL et al. (2007) 2006 Bethesda International Consensus recommendations on the immunophenotypic analysis of hematolymphoid neoplasia by flow cytometry: optimal reagents and reporting for the flow cytometric diagnosis of hematopoietic neoplasia. Cytometry B Clin. Cytom. 72: 14-22