Clone:
REAL444
Type of antibody:
Releasable fluorochromes, Primary antibodies, Recombinant antibodies
Applications:
MICS, IHC, IF
Alternative names:
TFRC, T9, TfR, TfR1, Trfr, p90

Specifications for CD71 Antibody, anti-human, REAdye_lease™

Overview

Clone REAL444 is an antibody fragment derived from the full CD71 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAdye_lease Complex to bind markers with high avidity.
Clone REAL444 recognizes the human CD71 antigen, a transferrin receptor, which is essential for iron transport into proliferating cells. CD71 is expressed on marrow stromal cells (MSCs) from bone marrow, on activated T and B lymphocytes, macrophages, and all proliferating cells. It is upregulated on lymphocytes during proliferative responses to antigens or mitogens but is not expressed on resting lymphocytes. CD71 is present on reticulocytes and erythroid progenitors in fetal liver, cord blood, and peripheral blood and may be used for determination of erythroid leukemia, benign erythroid proliferative disorders, and myelodysplastic syndrome.
CD71/transferrin receptor is apply for detecting erythropoietin (EPO) abuse by athletes and as a diagnostic test for anemia, e.g. resulting from rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV.
For removal of REAdye_lease fluorochromes for optional relabeling with different fluorochrome-conjugated REAdye_lease antibodies use the REAlease Support Kit (130-120-675).

Alternative names

TFRC, T9, TfR, TfR1, Trfr, p90

Detailed product information

Technical specifications

CloneREAL444
Clonalitymonoclonal
Isotype controlControl Antibody
Hostcell line
Type of antibodyReleasable fluorochromes, Primary antibodies, Recombinant antibodies
Specieshuman
AntigenCD71
Alternative names of antigenTFRC, T9, TfR, TfR1, Trfr, p90
Distribution of antigenB cells, endothelial cells, macrophages, T cells, other, ES and iPS cells, mesenchymal stem cells, hematopoietic stem and progenitor cells, other, spleen, liver, brain
RRIDAB_2857699

References for CD71 Antibody, anti-human, REAdye_lease™

Publications

  1. Judd, W. et al. (1980) Novel surface antigen expressed on dividing cells but absent from nondividing cells. J. Exp. Med. 152: 1430-1435
  2. Phillips, J. H. et al. (1984) Natural killer cells activated in a human mixed lymphocyte response culture identified by expression of Leu-11 and class II histocompatibility antigens. J. Exp. Med. 159: 993-1008
  3. Loken, M. R. et al. (1987) Flow cytometric analysis of human bone marrow: I. Normal erythroid development. Blood 69: 255-263
  4. Pittenger, M. F. et al. (1999) Multilineage potential of adult human mesenchymal stem cells. Science 284: 143-147
  5. Luchini, C. et al. (2016) CD71 in Gestational Pathology: A Versatile Immunohistochemical Marker With New Possible Applications. Appl. Immunohistochem. 24(3): 215-220