Clone:
REA414
Type of antibody:
Recombinant antibodies
Isotype:
recombinant human IgG1

Specifications for CD66c antibodies

Overview

REA414 recognizes the human CD66c antigen, a glycosylphosphatidylinositol (GPI) linked protein, which is also known as carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6). Within the hematopoietic system, CD66c expression is limited to granulocytes and its precursors, where it serves homotypic and heterotypic adhesion, Ca2
+
mediated signaling, and is markedly upregulated from intracellular stores after activation. It is also found in epithelia of various organs. CD66c overexpression is found in a wide variety of epithelial cancer types such as lung, breast, colorectal, and hepatocellular carcinomas.
Additional information: Clone REA414 displays negligible binding to Fc receptors.

Applications

Reagent can be used for immunophenotyping by flow cytometry. Abnormal numbers of cells expressing this antigen or aberrant expression levels of the antigen can be expected in some disease states. It is important to understand the normal expression pattern for this antigen and its relationship to expression of other relevant antigens in order to perform appropriate analysis.
Expression of CD66c may be used as aid to diagnostic in the characterization of samples from individuals suspected with hematologic neoplasia.

Detailed product information

Technical specifications

CloneREA414
Isotyperecombinant human IgG1
Type of antibodyRecombinant antibodies
Specieshuman
AntigenCD66c
Molecular mass of antigen [kDa]31
Distribution of antigenstem cells, granulocytes, other

Resources for CD66c antibodies

Certificates

Please follow this
link
to download the Certificate of Conformity (CoC) by lot number.

References for CD66c antibodies

Publications

  1. Stelzer GT et al. (1997) U.S.-Canadian consensus recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry: standardization and validation of laboratory procedures. Cytometry 30(5): 214-230
  2. Neumaier, M. et al. (1988) Characterization of a cDNA clone for the nonspecific cross-reacting antigen (NCA) and a comparison of NCA and carcinoembryonic antigen. J. Biol. Chem. 263(7): 3202-3207
  3. Rothe, G et al. (2012) Consensus protocol for the flow cytometric Immunophenotyping of hematopoietic malignancies. Leukemia 10(5): 877-895
  4. van Dongen, J. J. M. et al. (2012) EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia 26(9): 1908-1975
  5. Guillaume, N. et al. (2011) CD66c expression in B-cell acute lymphoblastic leukemia: strength and weakness. Int J Lab Hematol 33(1): 92-96
  6. Clinical and Laboratory Standards Institute (CLSI) (2007) Clinical Flow Cytometric Analysis of Neoplastic Hematolympoid Cells, CLSI document H43-A2 (ISBN 1-56238-635-2) CLSI; Approved Guideline - Second Edition
  7. Clinical and Laboratory Standards Institute (CLSI) (2007) Enumeration of immunologically defined cell populations by flow cytometry, CLSI document H42-A2 (ISBN 1-56238-640-9) CLSI; Approved Guideline - Second Edition
  8. Kiyokawa, N. et al. (2014) Significance of CD66c expression in childhood acute lymphoblastic leukemia. Leuk. Res. 38(1): 42-48