Clone:
REA889
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
CEACAM1, CEACAM5, CEACAM6

Extended validation for CD66ace Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA889
ASL-32++
Cells were incubated with an excess of purified unconjugated CD66ace (REA889) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD66ace. HT-29 cells were stained with CD66ace antibodies and plotted against the side scatter. As a control, CD66ace antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD66ace. HT-29 cells were stained with CD66ace antibodies and plotted against the side scatter. As a control, CD66ace antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD66ace. HT-29 cells were stained with CD66ace antibodies and plotted against the side scatter. As a control, CD66ace antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD66ace. HT-29 cells were stained with CD66ace antibodies and plotted against the side scatter. As a control, CD66ace antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD66ace (REA889). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD66ace (REA889). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD66ace (REA889). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD66ace Antibody, anti-human, REAfinity™

Overview

Clone REA889 recognizes the a, c, and e epitopes of the human CD66 antigen. CD66 constitutes a gene family whose members belong to the Ig superfamily of carcinoembryonic antigens (CEA). They are involved in a wide variety of physiological processes such as embryonic development, pregnancy, bile transport, as well as pathological conditions such as cancer, bacterial or viral infection, and inflammation. CD66a (CEACAM1), CD66c (CEACAM6), and CD66e (CEACAM5) are differentially expressed on some epithelial cells as well as on T cells, NK cell subsets, and granulocytes, especially neutrophils.
Additional information: Clone REA889 displays negligible binding to Fc receptors.

Alternative names

CEACAM1, CEACAM5, CEACAM6

Detailed product information

Technical specifications

CloneREA889
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD66ace
Alternative names of antigenCEACAM1, CEACAM5, CEACAM6
Molecular mass of antigen [kDa]52
Distribution of antigenT cells, NK cells, granulocytes
Entrez Gene ID634, 1048, 468
RRIDAB_2726654, AB_2726740, AB_2726655, AB_2726741, AB_2726656, AB_2726742, AB_2726657, AB_2726658, AB_2726653, AB_2726739

References for CD66ace Antibody, anti-human, REAfinity™

Publications

  1. Gray-Owen, S. D. and Blumberg, R. S. (2006) CEACAM1: contact-dependent control of immunity. Nat. Rev. Immunol. 6: 433-446
  2. Guillaume, N. et al. (2011) CD66c expression in B-cell acute lymphoblastic leukemia: strength and weakness. Int J Lab Hematol 33(1): 92-96

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