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Human peripheral blood cells after erythrocyte lysis were stained with CD65 antibodies as well as with CD16 antibodies. The specificity of the conjugated antibodies was confirmed by blocking the binding to the ligand, using pure unconjugated antibodies (left image). Flow cytometry was performed using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Other clones | Overlap in epitope recognition with VIM8 |
---|---|
REA1128 | ++ |
Cells were incubated with an excess of purified unconjugated CD65 (VIM8) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker. |
Clone | VIM8 |
---|---|
Clonality | monoclonal |
Isotype | mouse IgM |
Isotype control | Isotype Control Antibody, mouse IgM |
Host | mouse |
Type of antibody | Primary antibodies |
Species | human |
Antigen | CD65 |
Alternative names of antigen | VIM-2 |
Distribution of antigen | granulocytes, macrophages, monocytes, stem cells |
RRID | AB_2658949, AB_2658950, AB_2658951, AB_2658954, AB_2658955, AB_2658956, AB_2658957, AB_2658948 |
Other clones | Overlap in epitope recognition with VIM8 |
---|---|
REA1128 | ++ |
Cells were incubated with an excess of purified unconjugated CD65 (VIM8) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker. |
Clone | VIM8 |
---|---|
Clonality | monoclonal |
Isotype | mouse IgM |
Isotype control | Isotype Control Antibody, mouse IgM |
Host | mouse |
Type of antibody | Primary antibodies |
Species | human |
Antigen | CD65 |
Alternative names of antigen | VIM-2 |
Distribution of antigen | granulocytes, macrophages, monocytes, stem cells |
RRID | AB_2658949, AB_2658950, AB_2658951, AB_2658954, AB_2658955, AB_2658956, AB_2658957, AB_2658948 |
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