Name: CD65 Antibody, anti-human
Availability: InStock

Flow cytometry

Conjugate:	FITC
Recommended dilution:	1:11
Remark:	Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
Tested:	QC tested
Products used:	130-108-262, 130-108-235

Specifications for CD65 Antibody, anti-human

Overview

Clone VIM8 recognizes the human CD65 antigen, a fucosylated carbohydrate on the cell surface, which is also known as ceramide dodecasaccharide or VIM-2. CD65 is formed by fucosylation of the inner lactosamine unit of α-2,3-sialylated polylactosamine by α-1,3-fucosyltransferase. It is expressed on all myeloid cells during development, highly on granulocytes and weakly on monocytes in peripheral blood. The sialylated form of CD65 (designated CD65s) appears when the progenitor antigen CD34 disappears, suggesting that this sialylated carbohydrate antigen marks a turning point in normal myeloid differentiation. CD65 is a critical adhesion molecule for extravascular acute myeloid leukemia cell infiltration.

Alternative names

VIM-2

Detailed product information

Technical specifications

Clone	VIM8
Clonality	monoclonal
Isotype	mouse IgM
Isotype control	Isotype Control Antibody, mouse IgM
Host	mouse
Type of antibody	Primary antibodies
Species	human
Antigen	CD65
Alternative names of antigen	VIM-2
Distribution of antigen	granulocytes, macrophages, monocytes, stem cells
RRID	AB_2658949, AB_2658950, AB_2658951, AB_2658952, AB_2658953, AB_2658954, AB_2658955, AB_2658956, AB_2658957, AB_2658948

References for CD65 Antibody, anti-human

Publications

Lund-Johansen, F. et al. (1992) Activation of human phagocytes through carbohydrate antigens (CD15, sialyl-CD15, CDw17, and CDw65). J. Immunol. 148(10): 3221-3229
Bordessoule, D. et al. (1993) Immunohistological patterns of myeloid antigens: tissue distribution of CD13, CD14, CD16, CD31, CD36, CD65, CD66 and CD67. Br. J. Haematol. 83(3): 370-383
Kniep, B. et al. (1996)
The CDw65 monoclonal antibodies VIM-8 and VIM-11 bind to the neutral glycolipid V
³
FucnLc
₈
Cer.
J. Biochem. 119(3): 456-462

Applications

Specifications

Specifications for CD65 Antibody, anti-human

Overview

Alternative names

VIM-2

Detailed product information

Technical specifications

Clone	VIM8
Clonality	monoclonal
Isotype	mouse IgM
Isotype control	Isotype Control Antibody, mouse IgM
Host	mouse
Type of antibody	Primary antibodies
Species	human
Antigen	CD65
Alternative names of antigen	VIM-2
Distribution of antigen	granulocytes, macrophages, monocytes, stem cells
RRID	AB_2658949, AB_2658950, AB_2658951, AB_2658952, AB_2658953, AB_2658954, AB_2658955, AB_2658956, AB_2658957, AB_2658948

References

References for CD65 Antibody, anti-human

Publications

Lund-Johansen, F. et al. (1992) Activation of human phagocytes through carbohydrate antigens (CD15, sialyl-CD15, CDw17, and CDw65). J. Immunol. 148(10): 3221-3229
Bordessoule, D. et al. (1993) Immunohistological patterns of myeloid antigens: tissue distribution of CD13, CD14, CD16, CD31, CD36, CD65, CD66 and CD67. Br. J. Haematol. 83(3): 370-383
Kniep, B. et al. (1996)
The CDw65 monoclonal antibodies VIM-8 and VIM-11 bind to the neutral glycolipid V
³
FucnLc
₈
Cer.
J. Biochem. 119(3): 456-462

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CD65 Antibody, anti-human

CD65 Antibody, anti-human

Flow cytometry

Specifications for CD65 Antibody, anti-human

Overview

Alternative names

Detailed product information

Technical specifications

References for CD65 Antibody, anti-human

Publications

Specifications for CD65 Antibody, anti-human

Overview

Alternative names

Detailed product information

Technical specifications

References for CD65 Antibody, anti-human

Publications

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CD65 Antibody, anti-human