Clone:
VIM8
Type of antibody:
Primary antibodies
Isotype:
mouse IgM
Applications:
FC, MICS, IF, IHC
Alternative names:
VIM-2

Extended validation for CD65 Antibody, anti-human

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with VIM8
REA1128++
Cells were incubated with an excess of purified unconjugated CD65 (VIM8) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Specifications for CD65 Antibody, anti-human

Overview

Clone VIM8 recognizes the human CD65 antigen, a fucosylated carbohydrate on the cell surface, which is also known as ceramide dodecasaccharide or VIM-2. CD65 is formed by fucosylation of the inner lactosamine unit of α-2,3-sialylated polylactosamine by α-1,3-fucosyltransferase. It is expressed on all myeloid cells during development, highly on granulocytes and weakly on monocytes in peripheral blood. The sialylated form of CD65 (designated CD65s) appears when the progenitor antigen CD34 disappears, suggesting that this sialylated carbohydrate antigen marks a turning point in normal myeloid differentiation. CD65 is a critical adhesion molecule for extravascular acute myeloid leukemia cell infiltration.

Alternative names

VIM-2

Detailed product information

Technical specifications

CloneVIM8
Clonalitymonoclonal
Isotypemouse IgM
Isotype controlIsotype Control Antibody, mouse IgM
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman
AntigenCD65
Alternative names of antigenVIM-2
Distribution of antigengranulocytes, macrophages, monocytes, stem cells
RRIDAB_2658949, AB_2658950, AB_2658951, AB_2658952, AB_2658953, AB_2658954, AB_2658955, AB_2658956, AB_2658957, AB_2658948

References for CD65 Antibody, anti-human

Publications

  1. Lund-Johansen, F. et al. (1992) Activation of human phagocytes through carbohydrate antigens (CD15, sialyl-CD15, CDw17, and CDw65). J. Immunol. 148(10): 3221-3229
  2. Bordessoule, D. et al. (1993) Immunohistological patterns of myeloid antigens: tissue distribution of CD13, CD14, CD16, CD31, CD36, CD65, CD66 and CD67. Br J Haematol 83(3): 370-383
  3. Kniep, B. et al. (1996)
    The CDw65 monoclonal antibodies VIM-8 and VIM-11 bind to the neutral glycolipid V
    3
    FucnLc
    8
    Cer.
    J. Biochem. 119(3): 456-462

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