Clone:
10.1.1
Type of antibody:
Primary antibodies
Isotype:
mouse IgG1κ
Applications:
FC, MICS, IF, IHC, ICC, MC
Alternative names:
FCGR1A, CD64A, FcR I, IGFR1, FcγRI

Extended validation for CD64 Antibody, anti-human

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with 10.1.1
10.1++
REA978++
Cells were incubated with an excess of purified unconjugated CD64 (10.1.1) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Specifications for CD64 Antibody, anti-human

Overview

Clone 10.1.1 reacts with human CD64, a high affinity Fc receptor for IgG. CD64 contains three Ig-like domains in its extracellular domain and binds both monomeric and aggregated IgG and contributes to antibody-dependent cellular cytotoxicity (ADCC), phagocytosis, and the clearance of immune complexes. It is constitutively expressed on macrophages, monocytes, and eosinophils and can be up-regulated on neutrophils during infection or after stimulation with IFN-γ and colony-stimulating factors G-CSF and GM-CSF.

Alternative names

FCGR1A, CD64A, FcR I, IGFR1, FcγRI

Detailed product information

Technical specifications

Clone10.1.1
Clonalitymonoclonal
Isotypemouse IgG1κ
Isotype controlIsotype Control Antibody, mouse IgG1
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman, non-human primate
Cross-reactivity
rhesus monkey (
Macaca mulatta
)
,
cynomolgus monkey (
Macaca fascicularis
)
,
chimpanzee (
Pan troglodytes
)
, baboon, capuchin monkey,
squirrel monkey (
Saimiri sciureus
)
AntigenCD64
Alternative names of antigenFCGR1A, CD64A, FcR I, IGFR1, FcγRI
Molecular mass of antigen [kDa]41
Distribution of antigendendritic cells, macrophages, monocytes, myeloid leukemia cells, eosinophils, neutrophils
Entrez Gene ID2209
RRIDAB_2819586, AB_2819607, AB_2819592, AB_2658939, AB_2857691, AB_2857679, AB_2658923, AB_2658928, AB_2658929, AB_2658930, AB_2658931, AB_2658932, AB_2658933, AB_2658934, AB_2658935, AB_2658936, AB_2658937, AB_2658938, AB_2819601

References for CD64 Antibody, anti-human

Publications

  1. Buckle, A. M. and Hogg, N. (1989) The effect of IFN-gamma and colony-stimulating factors on the expression of neutrophil cell membrane receptors. J. Immunol. 143(7): 2295-2301
  2. Shen, L. et al. (1987) Polymorphonuclear leukocyte function triggered through the high affinity Fc receptor for monomeric IgG. J. Immunol. 139(2): 534-538
  3. Herra, C. et al. (1996) Increased expression of Fc gamma receptors on neutrophils and monocytes may reflect ongoing bacterial infection. J. Med. Microbiol. 44(2): 135-140

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