Clone:
REA828
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
Sell, L-selectin, LECAM-1, Lnhr, Ly-22, Ly-m22, Lyam-1, Lyam1, LAM-1

Extended validation for CD62L Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA828
MEL14-H2.100++
MEL14++
95218-
Cells were incubated with an excess of purified unconjugated CD62L (REA828) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD62L. Splenocytes from C57BL/6 mice were stained with CD62L antibodies and with a suitable counterstaining. As a control, CD62L antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD62L. Splenocytes from C57BL/6 mice were stained with CD62L antibodies and with a suitable counterstaining. As a control, CD62L antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD62L. Splenocytes from C57BL/6 mice were stained with CD62L antibodies and with a suitable counterstaining. As a control, CD62L antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD62L. Splenocytes from C57BL/6 mice were stained with CD62L antibodies and with a suitable counterstaining. As a control, CD62L antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD62L. Splenocytes from C57BL/6 mice were stained with CD62L antibodies and with a suitable counterstaining. As a control, CD62L antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD62L (REA828). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD62L (REA828). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD62L (REA828). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD62L Antibody, anti-mouse, REAfinity™

Overview

Clone REA828 recognizes mouse CD62L, also known as L-selectin. CD62L is a member of the selectin family of cell adhesion molecules. CD62L is important for the homing of lymphocytes to peripheral lymph nodes through high endothelial venules. It also contributes to leukocyte emigration into acute inflammatory sites. CD62L is a glycoprotein expressed on the cell surface of most thymocytes, T cells, B cells, monocytes, dendritic cells, neutrophils, and eosinophils. It is highly expressed on naive T cells and down-regulated upon differentiation due to activation-dependent shedding. CD62L is also expressed on a subset of CCR7
high
CD44
high
central memory T cells and on CD4
+
CD25
+
regulatory T cells.
Additional information: Clone REA828 displays negligible binding to Fc receptors.

Alternative names

Sell, L-selectin, LECAM-1, Lnhr, Ly-22, Ly-m22, Lyam-1, Lyam1, LAM-1

Detailed product information

Technical specifications

CloneREA828
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenCD62L
Alternative names of antigenSell, L-selectin, LECAM-1, Lnhr, Ly-22, Ly-m22, Lyam-1, Lyam1, LAM-1
Molecular mass of antigen [kDa]38
Distribution of antigenB cells, granulocytes, Langerhans cells, lymphocytes, monocytes, NK cells, red blood cells, T cells, eosinophils, neutrophils, thymocytes, bone marrow, spleen
Entrez Gene ID20343
RRIDAB_2658857, AB_2658858, AB_2658859, AB_2658860, AB_2658861, AB_2658862, AB_2658863, AB_2658864, AB_2658865, AB_2658866, AB_2658867, AB_2658868, AB_2658869, AB_2658870, AB_2658871, AB_2658872, AB_2658873, AB_2658856

References for CD62L Antibody, anti-mouse, REAfinity™

Publications

  1. Siegelman, M. H. et al. (1990) The mouse lymph node homing receptor is identical with the lymphocyte cell surface marker Ly-22: role of the EGF domain in endothelial binding. Cell 61(4): 611-622
  2. Gallatin, W. M. et al. (2006) A cell-surface molecule involved in organ-specific homing of lymphocytes. J. Immunol. 177(1): 5-9

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