Clone:
REAL152
Type of antibody:
Releasable fluorochromes, Primary antibodies, Recombinant antibodies
Applications:
MICS, IHC, IF
Alternative names:
L-selectin, LAM-1, Leu-8, LECAM1, TQ1, gp90-MEL

Specifications for CD62L Antibody, anti-human, REAdye_lease™

Overview

Clone REAL152 is an antibody fragment derived from the full CD62L antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAdye_lease Complex to bind markers with high avidity.
Clone REAL152 recognizes the human CD62L antigen, a 74 kDa Single-pass type I membrane protein which is also known as L-selectin, LECAM-1 or LAM-1. CD62L is a member of the selectin family of cell surface molecules and binds a series of glycoproteins including CD34, GlyCAM-1, and MAdCAM-1. Most hematopoietic cells express CD62L, including peripheral blood B cells, T cells, monocytes, granulocytes, thymocytes, and some myeloid cells from bone marrow. Expression on resting naïve, central memory and some effector memory T cells regulates their migration via endothelial venules to peripheral lymph nodes and Peyer’s patches. The CD62L antigen also contributes to the recruitment of leukocytes from the blood to areas of inflammation. Disruption of CD62L expression has detrimental effects on T cell migration and immune responses. Antigenic activation in the lymph node leads first to rapid CD62L shedding by protease cleavage and then to transcriptional suppression.
For removal of REAdye_lease fluorochromes for optional relabeling with different fluorochrome-conjugated REAdye_lease antibodies use the REAlease Support Kit (130-120-675).

Alternative names

L-selectin, LAM-1, Leu-8, LECAM1, TQ1, gp90-MEL

Detailed product information

Technical specifications

CloneREAL152
Clonalitymonoclonal
Isotype controlControl Antibody
Hostcell line
Type of antibodyReleasable fluorochromes, Primary antibodies, Recombinant antibodies
Specieshuman
AntigenCD62L
Alternative names of antigenL-selectin, LAM-1, Leu-8, LECAM1, TQ1, gp90-MEL
Distribution of antigenB cells, T cells, monocytes, granulocytes, myeloid cells, other, neutrophils, regulatory T cells

References for CD62L Antibody, anti-human, REAdye_lease™

Publications

  1. Ivetic, A. (2013) Signals regulating L-selectin-dependent leucocyte adhesion and transmigration. Int. J. Biochem. Cell Biol. 45(3): 550-555
  2. McEver, R. P. (2015) Selectins: initiators of leucocyte adhesion and signalling at the vascular wall. Cardiovasc. Res. 107(3): 331-339
  3. Telen, M. J. (2014) Cellular adhesion and the endothelium: E-selectin, L-selectin, and pan-selectin inhibitors. Hematol. Oncol. Clin. North Am. 28(2): 341-354