Clone:
REA1192
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IHC, IF
Alternative names:
ITGB3, GP3A, INGRB3, GPIIIa, Integrin beta-3, Integrin β3

Extended validation for CD61 Antibody, anti-mouse/rat, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA1192
2C9.G2++
Cells were incubated with an excess of purified unconjugated CD61 (REA1192) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD61 (REA1192). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD61 (REA1192). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD61 (REA1192). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD61 (REA1192). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD61 (REA1192). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD61 Antibody, anti-mouse/rat, REAfinity™

Overview

Clone REA1192 recognizes a 110 kDa integrin β chain known as CD61 or integrin β3. Owing to multiple α/β subunit combinations, integrins form an array of heterodimeric receptors, each recognising a multitude of ligands. CD61, in association with either αv (CD51) or αIIb (CD41) integrin, binds to fibrinogen, fibronectin, von Willebrand factor, prothrombin, disintegrins, and thrombospondin. Due to binding to various extracellular matrix proteins and cellular receptors, integrins play a significant role in cell adhesion and cell movement. Furthermore, a pro-apoptotic function of CD61 has been suggested using a hepatocellular carcinoma (HCC) cell line and patient tissues. Expression of CD61 is found on platelets, B cell subsets, myeloid cells, mast cells, osteoclasts, endothelium, megakaryocytes, and smooth muscle cells.
Additional information: Clone REA1192 displays negligible binding to Fc receptors.

Alternative names

ITGB3, GP3A, INGRB3, GPIIIa, Integrin beta-3, Integrin β3

Detailed product information

Technical specifications

CloneREA1192
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse, rat
AntigenCD61
Alternative names of antigenITGB3, GP3A, INGRB3, GPIIIa, Integrin beta-3, Integrin β3
Molecular mass of antigen [kDa]84
Distribution of antigenB cell subsets, smooth muscle
Entrez Gene ID16416
RRIDAB_2801837, AB_2801838, AB_2801839, AB_2801840, AB_2801841, AB_2801836

Resources for CD61 Antibody, anti-mouse/rat, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD61 Antibody, anti-mouse/rat, REAfinity™

Publications

  1. Plow, E. F. et al. (2000) Ligand binding to integrins. J. Biol. Chem. 275: 21785-21788

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