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Mouse bone marrow suspension cells were stained with CD61 antibodies conjugated to (A) FITC, (B) PE, (C) APC, (D) PE-Vio770, and (E) Biotin as well as with Anti-Ter-119 antibodies conjugated to APC or PE and analyzed by flow cytometry using the MACSQuant® Analyzer. Cells labeled with CD61-Biotin were stained with Anti-Biotin-PE as well. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of CD61-PE-Vio770.
Other clones | Overlap in epitope recognition with 2C9.G2 |
---|---|
REA1192 | ++ |
Cells were incubated with an excess of purified unconjugated CD61 (2C9.G2 (HMβ3-1)) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker. |
Clone | 2C9.G2 (HMβ3-1) |
---|---|
Clonality | monoclonal |
Isotype | hamster IgGκ |
Host | hamster |
Type of antibody | Primary antibodies |
Species | mouse, rat |
Antigen | CD61 |
Alternative names of antigen | ITGB3, GP3A, INGRB3, GPIIIa |
Molecular mass of antigen [kDa] | 84 |
Distribution of antigen | B cells, endothelial cells, fibroblasts, macrophages, mast cells, megakaryocytes, microglia, platelets, CNS cells, smooth muscle |
Entrez Gene ID | 16416 |
RRID | AB_2658828, AB_2658829, AB_2658830, AB_2658831, AB_2658832, AB_2658833, AB_2658834, AB_2658835, AB_2658836, AB_2658837 |
Other clones | Overlap in epitope recognition with 2C9.G2 |
---|---|
REA1192 | ++ |
Cells were incubated with an excess of purified unconjugated CD61 (2C9.G2 (HMβ3-1)) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker. |
Clone | 2C9.G2 (HMβ3-1) |
---|---|
Clonality | monoclonal |
Isotype | hamster IgGκ |
Host | hamster |
Type of antibody | Primary antibodies |
Species | mouse, rat |
Antigen | CD61 |
Alternative names of antigen | ITGB3, GP3A, INGRB3, GPIIIa |
Molecular mass of antigen [kDa] | 84 |
Distribution of antigen | B cells, endothelial cells, fibroblasts, macrophages, mast cells, megakaryocytes, microglia, platelets, CNS cells, smooth muscle |
Entrez Gene ID | 16416 |
RRID | AB_2658828, AB_2658829, AB_2658830, AB_2658831, AB_2658832, AB_2658833, AB_2658834, AB_2658835, AB_2658836, AB_2658837 |
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